Expression of the non-neuronal cholinergic system in human knee synovial tissue from patients with rheumatoid arthritis and osteoarthritis
| Autor: | Hans-Georg Morhenn, Katrin S. Lips, Jan Schubert, Gabor Szalay, Reinhard Schnettler, Christian Heiss, Sonja Hartmann, Janet Beckmann |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
medicine.medical_specialty Knee Joint Arthritis Gene Expression Osteoarthritis Receptors Nicotinic General Biochemistry Genetics and Molecular Biology Arthritis Rheumatoid Internal medicine Muscarinic acetylcholine receptor Medicine Humans RNA Messenger General Pharmacology Toxicology and Pharmaceutics Receptor Acetylcholine receptor Aged business.industry Synovial Membrane General Medicine Middle Aged medicine.disease Choline acetyltransferase Receptors Muscarinic Acetylcholine Nicotinic agonist Endocrinology business medicine.drug |
| Zdroj: | Life sciences. 91(21-22) |
| ISSN: | 1879-0631 |
| Popis: | Aims As the stimulation of the α7-nicotinic acetylcholine receptor (nAChR), which is present in the synovium of patients with rheumatoid arthritis (RA), leads to a decrease in pro-inflammatory cytokines, the α7-nAChR is being discussed as a new therapeutic target. On this background we addressed the question whether α7-nAChR mRNA was differentially expressed in RA compared to osteoarthritis (OA) synovial samples and whether other components of the non-neuronal cholinergic system were also present and differentially expressed in the synovium of patients with RA in comparison to OA. Main methods The expression of nicotinic and muscarinic acetylcholine receptors (mAChRs), choline and acetylcholine transporters, synthesising and degrading enzymes was determined in human samples of synovial tissue from patients with RA and OA using RT-PCR and immunofluorescence labelling. Key findings Compared to OA, patients with RA showed increased expression of nAChR subunit β4 while a decline in subunits α2 and α4 as well as in mAChR M1R was observed. For all other nAChR subunits and mAChRs however there was no significant difference between RA and OA patients. With regard to the ACh transporters and enzymes no expressional changes were observed between OA and RA patients, except for the choline acetyltransferase (ChAT) which was only detected in OA but not in RA synovium. Significance Our results indicate that besides α7-nAChR other components of the non-neuronal cholinergic system are present and differentially expressed in the synovium of RA and OA patients, which makes them interesting alternative targets in the development of new strategies for RA therapy. |
| Databáze: | OpenAIRE |
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