Survival of Cryptosporidium parvum oocysts recovered from experimentally contaminated oysters (Ostrea edulis) and clams (Tapes decussatus)
| Autor: | M E Ares-Mazás, A.M Oteiza-López, F. Freire-Santos, Hipólito Gómez-Couso, M R Ortega-Iñarrea, J. A. Castro-Hermida, Oscar Garcia-Martin |
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| Rok vydání: | 2002 |
| Předmět: |
Oyster
Food Chain animal diseases Microbiology Host-Parasite Interactions Apicomplexa Mice biology.animal parasitic diseases Animals Ostrea edulis Mollusca Parasite Egg Count Shellfish Infectivity Cryptosporidium parvum General Veterinary biology fungi Oocysts General Medicine biology.organism_classification Bivalvia Ostreidae Infectious Diseases Insect Science Parasitology Cattle Water Microbiology |
| Zdroj: | Parasitology research. 88(2) |
| ISSN: | 0932-0113 |
| Popis: | Samples of two species of shellfish that form part of the human food chain (the oyster Ostrea edulis and the marine clam Tapes decussatus) were experimentally contaminated with Cryptosporidium parvum oocysts. Changes in the viability of oocysts subsequently recovered from the shellfish were evaluated by means of an immunofluorescent antibody technique (IFAT) and inclusion/exclusion of the fluorogenic vital dye propidium iodide. There was a sharp decrease in oocyst viability during the first 4 days, with 15-25% viable oocysts remaining thereafter. In addition the infectivity of these oocysts at 10 and 31 days post-contamination was demonstrated using a suckling murine model. |
| Databáze: | OpenAIRE |
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