Membrane reshaping by micrometric curvature sensitive septin filaments
| Autor: | Manuela Nania, Patricia Bassereau, Hervé Isambert, Aurélie Di Cicco, Feng-Ching Tsai, Alexandre Beber, Daniel Lévy, João T. Cabral, Stéphanie Mangenot, Cyntia Taveneau, Aurélie Bertin |
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| Přispěvatelé: | Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Imperial College London |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
MECHANISM Lipid Bilayers General Physics and Astronomy 02 engineering and technology CYTOSKELETON Septin Cell membrane lcsh:Science Cytoskeleton Lipid bilayer Multidisciplinary PLASMA Vesicle 021001 nanoscience & nanotechnology DIFFUSION Multidisciplinary Sciences Membrane medicine.anatomical_structure Science & Technology - Other Topics biological phenomena cell phenomena and immunity Structural biology 0210 nano-technology Cell Division Materials science Saccharomyces cerevisiae Proteins Science [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Biophysics macromolecular substances ORGANIZATION Saccharomyces cerevisiae Curvature Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Imaging Three-Dimensional MD Multidisciplinary medicine Unilamellar Liposomes Science & Technology fungi ELASTICITY Cell Membrane General Chemistry Models Theoretical BARRIER 030104 developmental biology Microscopy Fluorescence CYTOKINESIS lcsh:Q Biological physics Cytokinesis Septins |
| Zdroj: | Nature Communications Nature Communications, Nature Publishing Group, 2019, 10 (1), pp.420. ⟨10.1038/s41467-019-08344-5⟩ Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2041-1723 |
| Popis: | Septins are cytoskeletal filaments that assemble at the inner face of the plasma membrane. They are localized at constriction sites and impact membrane remodeling. We report in vitro tools to examine how yeast septins behave on curved and deformable membranes. Septins reshape the membranes of Giant Unilamellar Vesicles with the formation of periodic spikes, while flattening smaller vesicles. We show that membrane deformations are associated to preferential arrangement of septin filaments on specific curvatures. When binding to bilayers supported on custom-designed periodic wavy patterns displaying positive and negative micrometric radii of curvatures, septin filaments remain straight and perpendicular to the curvature of the convex parts, while bending negatively to follow concave geometries. Based on these results, we propose a theoretical model that describes the deformations and micrometric curvature sensitivity observed in vitro. The model captures the reorganizations of septin filaments throughout cytokinesis in vivo, providing mechanistic insights into cell division. Septins are cytoskeletal filaments that localize at constriction sites and impact membrane remodeling. Here authors examine the curvature sensitivity of septins using bilayers on wavy patterns and derive a theoretical model that quantitatively describe the results. |
| Databáze: | OpenAIRE |
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