Extraction of [14CI Vitamin a from Rat Liver and Kidney During Processing for Transmission Electron Microscopy
| Autor: | D. L. Wallace, Charles G. Plopper, J. D. Pietzsch |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Male
Vitamin medicine.medical_specialty Pathology Kidney Specimen Handling law.invention chemistry.chemical_compound law Internal medicine medicine Animals Propylene oxide Vitamin A Ethanol Retinol Osmium Lipids Rats Microscopy Electron Endocrinology medicine.anatomical_structure Liver chemistry Osmium tetroxide Glutaraldehyde Anatomy Electron microscope |
| Zdroj: | Stain Technology. 50:339-345 |
| ISSN: | 0038-9153 |
| DOI: | 10.3109/10520297509117084 |
| Popis: | The retention of radio isotope-labekd vitamin A during processing for electron microscopy was investigated using the livers and kidneys of vitamin A deficient rats. [15-14C]Retinol (3μCi/animal) was administered by esophageal intubation to male rats which had been maintained on a vitamin A deficient diet for five or sir weeks postweaning. Glutaraldehyde- or osmium-fixed tissue was processed by three methods: a) routine (a graded series of ethanols, propylene oxide and epoxy), b) rapid (75% and 95% ethanol with three changes of epoxy), or c) water-soluble embedding (70% and 80% hydrorypropyl methacrylate). Water-soluble embedding retained the highest percentage of label in the tissue (liver: 96.31%; kidney: 98.68%). Inclusion of osmium tetroxide in the processing sequence and minimal exposure of tissue to lipid solvents were necessary for good retention of labeled vitamin A in tissues.The opinions or assertions contained herein are the private views of the author and are not to be construed as official or ... |
| Databáze: | OpenAIRE |
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