A modified ex vivo skin organ culture system for functional studies
Autor: | Jon D. Laman, Errol P. Prens, Liu Wei, L. I. van der Wel, Arjen Companjen |
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Rok vydání: | 2001 |
Předmět: |
Lipopolysaccharides
Pathology medicine.medical_specialty CD3 Complex medicine.medical_treatment Dermatology Organ culture Dexamethasone Interferon-gamma Tissue culture Organ Culture Techniques Antigens CD In vivo medicine Humans Skin equivalent CD40 Antigens Glucocorticoids Skin Membrane Glycoproteins CD40 integumentary system biology HLA-DR Antigens General Medicine Intercellular Adhesion Molecule-1 Cell biology Cytokine biology.protein Cytokines Female Cytokine secretion B7-2 Antigen Ex vivo Interleukin-1 |
Zdroj: | Archives of Dermatological Research. 293:184-190 |
ISSN: | 1432-069X 0340-3696 |
Popis: | To investigate the immunological function of cells in normal and diseased skin under conditions approximating the in vivo situation, it is necessary to maintain the structural integrity of the tissue. To achieve this, freshly isolated skin has to be cultured ex vivo, or an in vitro-constructed complete skin equivalent may be used. Different skin organ culture systems have been described. Basically two systems prevail: submerged or air-exposed skin organ cultures. The former model has been used for measuring cytokine secretion by skin cells in the medium, and the latter to study the expression of cell membrane proteins in situ and the kinetics of epidermal Langerhans cells. Here we present a modified ex vivo skin organ culture system which approaches the in vivo situation by maintaining the normal skin architecture without spontaneous induction of the regenerative maturation markers. This method allowed the expression of cell membrane proteins in situ to be measured, and the cytokine secretion by skin cells in the culture medium to be quantitated in the same experiment. In this system, both general and specific stimuli (LPS and IL-1beta) upregulated the expression of skin-derived cytokines IL-8 and IL-6 in the medium and different markers (ICAM-1, CD40 and CD86) on cells in the tissue in a 24-hour culture-formed. Elevation of both cytokine and cell marker expression could be blocked by dexamethasone and by IL-1ra which acts specifically on IL-1beta-mediated responses. The system presented here is both quick and simple and can be used as a model to study the behaviour of skin cells in their natural microenvironment. |
Databáze: | OpenAIRE |
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