Activated whole-body arginine pathway in high-active mice
| Autor: | J. Timothy Lightfoot, John J. Thaden, Jorge Z. Granados, Gabriella A. M. Ten Have, Ayland C. Letsinger, Nicolaas E. P. Deutz, Mariëlle P.K.J. Engelen |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Arginine Physiology Phenylalanine 030204 cardiovascular system & hematology Biochemistry Mice chemistry.chemical_compound 0302 clinical medicine Animal Cells Tandem Mass Spectrometry Blood plasma Medicine and Health Sciences Citrulline Amino Acids Protein Metabolism Neurons Mice Inbred C3H Mammalian Genomics Multidisciplinary Organic Compounds Neurochemistry Genomics Ornithine Body Fluids Chemistry Blood Physical Sciences Medicine Metabolic Pathways Neurochemicals Cellular Types Basic Amino Acids Anatomy Research Article medicine.medical_specialty Science Motor Activity Nitric Oxide Creatine Olfactory Receptor Neurons Blood Plasma 03 medical and health sciences Physical Conditioning Animal Internal medicine Genetics medicine Animals Organic Chemistry Chemical Compounds Biology and Life Sciences Afferent Neurons Proteins Cell Biology Metabolism Mice Inbred C57BL Glutamine 030104 developmental biology Endocrinology chemistry Animal Genomics Cellular Neuroscience Neuroscience Chromatography Liquid |
| Zdroj: | PLoS ONE, Vol 15, Iss 6, p e0235095 (2020) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Our previous studies suggest that physical activity (PA) levels are potentially regulated by endogenous metabolic mechanisms such as the vasodilatory roles of nitric oxide (NO) production via the precursor arginine (ARG) and ARG-related pathways. We assessed ARG metabolism and its precursors [citrulline (CIT), glutamine (GLN), glutamate (GLU), ornithine (ORN), and phenylalanine (PHE)] by measuring plasma concentration, whole-body production (WBP), de novo ARG and NO production, and clearance rates in previously classified low-active (LA) or high-active (HA) mice. We assessed LA (n = 23) and HA (n = 20) male mice by administering a stable isotope tracer pulse via jugular catheterization. We measured plasma enrichments via liquid chromatography tandem mass spectrometry (LC-MS/MS) and body compostion by echo-MRI. WBP, clearance rates, and de novo ARG and NO were calculated. Compared to LA mice, HA mice had lower plasma concentrations of GLU (71.1%; 36.8 ± 2.9 vs. 17.5 ± 1.7μM; p |
| Databáze: | OpenAIRE |
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