Expression of an A20/AN1 Stress-Associated Protein from Aeluropus littoralis in Rice Deregulates Stress-Related Genes

Autor: Jean-Benoit Morel, Ahmed Abdelrahim Mohamed Ali, Afif Hassairi, Nabil Zouari, Donaldo Meynard, Mohamed Tarroum, Christophe Perin, Gaëtan Droc, Lotfi Fki, Rania Ben Saad, Walid Ben Romdhane, Emmanuel Guiderdoni, Abdullah A. Al-Doss
Přispěvatelé: King Saud University [Riyadh] (KSU), Université de Sfax - University of Sfax, Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Biologie et Génétique des Interactions Plantes-Agents Pathogènes, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure Agronomique de Montpellier (ENSA M)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Plant Health Institute of Montpellier (UMR PHIM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), National Plan for Science, Technology and Innovation (MAARIFAH), King Abdul Aziz City for Science and Technology, Kingdom of Saudi Arabia (Award number 2- 17-04-001-0046).
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Journal of Plant Growth Regulation
Journal of Plant Growth Regulation, Springer Verlag, In press, ⟨10.1007/s00344-021-10344-z⟩
ISSN: 0721-7595
1435-8107
DOI: 10.1007/s00344-021-10344-z⟩
Popis: International audience; The AlSAP gene, which has been isolated from the C4 halophyte grass Aeluropus littoralis, a member of the stress-associated protein gene family. Like other members of this family, AlSAP contains A20 and AN1 zinc-finger domains, and its transcription is induced by abiotic stresses such as salinity, drought, and temperature as well as by several hormones, including abscisic and salicylic acids. Additionally, AlSAP overexpression in rice has been demonstrated to enhance cold and drought stress tolerance. In the present study, we investigated the transcriptional deregulation of stress-related genes following ectopic expression of the AlSAP gene in two transgenic rice lines, namely, RN4 and RN5. Compared with control plants, a total of 1734 and 3650 annotated genes were found to be differentially expressed in the leaves and roots of the RN4 and RN5 lines, respectively. AlSAP transcript accumulation primed the expression of abiotic-stress-related genes involved in transcription, signaling, protein degradation, and hormonal homeostasis in rice plants grown under unstressed conditions. We hypothesized that this priming underlies the enhanced tolerance to abiotic stresses previously reported in AlSAP rice lines. Notably, AlSAP also induces transcript accumulation of genes known to be major negative regulators of pathogen response in rice. Consistently, our results showed that abiotic stress-tolerant AlSAP rice lines also exhibit enhanced susceptibility to rice blast fungus Magnaporthe oryzae.
Databáze: OpenAIRE
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