Ultrasonic-augmented Primary Adult Fibroblast Isolation
| Autor: | Susanne Kämmerer, Charlotte Schaeffer, Carola S. Mehnert, Stephanie M Schacht Wall, Karolina Sekeres, Ali El-Armouche, Stephan R Künzel, Manja Newe |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Senescence Cellular differentiation General Chemical Engineering Cell Inflammation Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Mice 0302 clinical medicine Fibrosis medicine Animals Ultrasonics Fibroblast Cells Cultured General Immunology and Microbiology General Neuroscience Cell Differentiation Fibroblasts medicine.disease Cell biology 030104 developmental biology medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis medicine.symptom Myofibroblast |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| Popis: | Primary adult fibroblasts have become an important tool to study fibrosis, fibroblast interactions and inflammation in all body tissues. Since primary fibroblasts cannot divide indefinitely due to myofibroblast differentiation or senescence induction, new cultures must be established regularly. However, there are several obstacles to overcome during the processes of developing a reliable isolation protocol and primary fibroblast isolation itself: the method's degree of difficulty (especially for beginners), the risk of bacterial contamination, the required time until primary fibroblasts can be used for experiments, and subsequent cell quality and viability. In this study, a fast, reliable and easy-to-learn protocol to isolate and culture primary adult fibroblasts from mouse heart, lung, liver and kidney combining enzymatic digestion and ultrasonic agitation is provided. |
| Databáze: | OpenAIRE |
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