HOTAIR long non-coding RNA is a negative prognostic factor not only in primary tumors, but also in the blood of colorectal cancer patients
| Autor: | Vaclav Liska, Michaela Schneiderova, Jana Slyskova, Peter J. Makovicky, Beáta Hemmelová, Lucie Schwarzova, Miroslav Svoboda, Ludmila Lipska, Zdenek Kala, Ludovit Bielik, Ludmila Vodickova, Ondrej Vycital, Markéta Protivánková, Miroslav Levy, Pavel Vodicka |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Oncology Cancer Research medicine.medical_specialty Pathology Colon Colorectal cancer Biology Real-Time Polymerase Chain Reaction Internal medicine Biomarkers Tumor medicine Humans RNA Messenger Stage (cooking) Survival rate Aged Neoplasm Staging Aged 80 and over Reverse Transcriptase Polymerase Chain Reaction Hazard ratio Rectum Case-control study Microsatellite instability HOTAIR General Medicine Middle Aged Prognosis medicine.disease 3. Good health Survival Rate Real-time polymerase chain reaction Case-Control Studies Female RNA Long Noncoding Neoplasm Grading Colorectal Neoplasms Follow-Up Studies |
| Zdroj: | Carcinogenesis. 35:1510-1515 |
| ISSN: | 1460-2180 0143-3334 |
| Popis: | Colorectal cancer (CRC) is one of the main causes of death of neoplasia. Demand for predictive and prognostic markers to reverse this trend is increasing. Long non-coding RNA HOTAIR (Homeobox Transcript Antisense Intergenic RNA) overexpression in tumors was previously associated with poor prognosis and higher mortality in different carcinomas. We analyzed HOTAIR expression levels in tumor and blood of incident sporadic CRC patients in relation to their overall survival with the aim to evaluate surrogate prognostic marker for CRC. Tissue donor group consisted of 73 CRC patients sampled for tumor and normal tissue. Blood donor group was represented by 84 CRC patients compared with 40 healthy controls. Patients were characterized for tumor-node-metastasis stage, tumor grade, microsatellite instability and tumor penetration by stromal cells. HOTAIR levels were assessed by real-time quantitative PCR. CRC patients had higher HOTAIR expression in blood than healthy controls (P = 0.0001), whereas there was no difference in HOTAIR levels between tumor and adjacent mucosa of CRC patients. HOTAIR levels positively correlated between blood and tumor (R = 0.43, P = 0.03). High HOTAIR levels in tumors were associated with higher mortality of patients [Cox's proportional hazard, hazard ratio = 4.4, 95% confidence interval: 1.0-19.2, P = 0.046]. The hazard ratio was even higher when blood HOTAIR levels were taken into account (hazard ratio = 5.9, 95% confidence interval: 1.3-26.1, P = 0.019). Upregulated HOTAIR relative expression in primary tumors and in blood of CRC patients is associated with unfavorable prognosis. Our data suggest that HOTAIR blood levels may serve as potential surrogate prognostic marker in sporadic CRC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|