Multiphasic modulation of signal transduction into T lymphocytes by monoiodoacetic acid as a sulfhydryl reagent
| Autor: | Mei-yi Pu, Naoko Ohata, Takashi Iwamoto, Michinari Hamaguchi, Izumi Nakashima, K Ohkusu, Masashi Kato, Li Ma, Hong Yi, Anwarul A. Akhand, Ken-ichi Isobe |
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| Rok vydání: | 1995 |
| Předmět: |
CD3 Complex
Transcription Genetic endocrine system diseases T-Lymphocytes T cell CD3 Iodoacetates Biochemistry Mice chemistry.chemical_compound Sulfhydryl reagent medicine Animals Phosphorylation Genes Immediate-Early Molecular Biology Cells Cultured biology Cell growth Chemistry Sulfhydryl Reagents T-cell receptor Receptors Interleukin-2 Tyrosine phosphorylation Cell Biology Molecular biology digestive system diseases Iodoacetic Acid Mice Inbred C57BL medicine.anatomical_structure biology.protein Interleukin-2 Tyrosine Signal transduction Cell activation Cell Division Signal Transduction |
| Zdroj: | Journal of Cellular Biochemistry. 59:33-41 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.240590105 |
| Popis: | Actions of monoiodoacetic acid (MIA) as a sulfhydryl reagent on the different stages of the T cell receptor (TCR)–mediated signal transduction were examined. MIA (1 mM) prevented anti-TCR (CD3) monoclonal antibody (mAb)–induced energy-dependent receptor capping but at the same time promoted the anti-CD3 mAb/mitogen-induced tyrosine phosphorylation of the T cell activation-linked cellular proteins of 120, 80, 70, 56, and 40 KDa. Relatively low concentration (0.01 mM) of MIA further promoted anti-CD3 mAb-induced transcription of c-fos, production of IL-2, and cell surface expression of IL-2 receptors. The MIA-promoted TCR-mediated IL-2 production actually required signal transduction that could be inhibited by cyclosporin A, genistein, or H-7. In contrast, the same concentration of MIA as promoted the signal transduction for cell activation severely inhibited the anti-CD3 mAb-triggered signal delivery for cell proliferation, selectively at its early stage. We conclude from these results that MIA differentially affects various steps of signaling into T lymphocytes, suggesting that there exist multiple sites of MIA-sensitive or redox-linked control in the signal cascade. © 1995 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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