Detection of tumor-associated antigens in culture supernatants using autoantibodiesin sera from patients with bladder cancer
| Autor: | Yuichi Sato, Masatsugu Iwamura, Ryo Nagashio, Manabu Hattori, Kazumasa Matsumoto, Junpei Ueda, Kazuo Hachimura, Sho Minami, Naoki Goshima |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Proteomics MMP2 medicine.disease_cause General Biochemistry Genetics and Molecular Biology Antigens Neoplasm Cell Line Tumor Biomarkers Tumor Humans Medicine Neoplasm Metastasis Aged Autoantibodies Neoplasm Staging Aged 80 and over Bladder cancer business.industry Autoantibody Reproducibility of Results General Medicine Middle Aged medicine.disease Patient Outcome Assessment Secretory protein Urinary Bladder Neoplasms Culture Media Conditioned Immunoglobulin G Immunology Matrix Metalloproteinase 2 Biomarker (medicine) Female Cell culture supernatant Cancer biomarkers Neoplasm Grading Calreticulin business Carcinogenesis |
| Zdroj: | Biomedical Research. 35:25-35 |
| ISSN: | 1880-313X 0388-6107 |
| DOI: | 10.2220/biomedres.35.25 |
| Popis: | Secreted proteins play essential roles in the process of tumorigenesis, and the analysis of tumor- secreted proteins has been suggested as a promising strategy for identifying cancer biomarkers. In this study, we performed proteomic analysis to identify proteins secreted from bladder cancer cell lines that are recognized by autoantibodies in sera from patients with bladder cancer. In addition, autoantibodies against the identified proteins were validated using a dot-blot array with sera from patients with bladder cancer and normal controls. As the results, we detected twenty-five and thir - ty-two immunoreactive spots in sera from patients with high- and low-grade bladder cancer, re- spectively. In addition, validation analysis revealed that serum IgG levels of anti-calreticulin and matrix metalloproteinase-2 (MMP2) autoantibodies were significantly higher in bladder cancer pa- tients than in normal controls (both P < 0.05). Furthermore, the serum IgG level of anti-MMP2 autoantibody was significantly higher in patients with high- compared to low-grade bladder cancer (P < 0.05). On multivariate analysis, the serum IgG level of anti-MMP2 autoantibody was an in- dependent predictor of cancer-specific survival ( P < 0.05). Based on these findings, serum IgG lev - els of anti-calreticulin and MMP2 autoantibodies may be novel biomarker candidates for bladder cancer and its clinical outcome. |
| Databáze: | OpenAIRE |
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