The transcription factor AtGLK1 acts upstream of MYBL2 to genetically regulate sucrose-induced anthocyanin biosynthesis in Arabidopsis
| Autor: | Fang Zhang, lingjun Yang, Ziyu Yao, Dong Liu, Haiyan Jiang, Yuxuan Zheng, Jianfeng Cheng, Dongming Zhao |
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| Rok vydání: | 2021 |
| Předmět: |
Sucrose
AtGLK1 Transgene Mutant Arabidopsis MYBL2 Plant Science Anthocyanins chemistry.chemical_compound Anthocyanin biosynthesis Transcription factor Regulator gene biology Arabidopsis Proteins Research fungi Botany food and beverages Plants Genetically Modified biology.organism_classification Cell biology DNA-Binding Proteins carbohydrates (lipids) Real-time polymerase chain reaction chemistry Seedlings QK1-989 Anthocyanin Signal Transduction Transcription Factors |
| Zdroj: | BMC Plant Biology, Vol 21, Iss 1, Pp 1-14 (2021) BMC Plant Biology |
| ISSN: | 1471-2229 |
| DOI: | 10.1186/s12870-021-03033-2 |
| Popis: | Background The regulation of anthocyanin biosynthesis by various factors including sugars, light and abiotic stresses is mediated by numerous regulatory factors acting at the transcriptional level. Here experimental evidence was provided in order to demonstrate that the nuclear GARP transcription factor AtGLK1 plays an important role in regulating sucrose-induced anthocyanin biosynthesis in Arabidopsis. Results The results obtained using real-time quantitative PCR and GUS staining assays revealed that AtGLK1 was mainly expressed in the green tissues of Arabidopsis seedlings and could be induced by sucrose. The loss-of-function glk1 glk2 double mutant has lower anthocyanin levels than the glk2 single mutant, although it has been determined that loss of AtGLK1 alone does not affect anthocyanin accumulation. Overexpression of AtGLK1 enhances the accumulation of anthocyanin in transgenic Arabidopsis seedlings accompanied by increased expression of anthocyanin biosynthetic and regulatory genes. Moreover, we found that AtGLK1 also participates in plastid-signaling mediated anthocyanin accumulations. Genetic, physiological, and molecular biological approaches demonstrated that AtGLK1 acts upstream of MYBL2, which is a key negative regulator of anthocyanin biosynthesis, to genetically regulate sucrose-induced anthocyanin biosynthesis. Conclusion Our results indicated that AtGLK1 positively regulates sucrose-induced anthocyanin biosynthesis in Arabidopsis via MYBL2. |
| Databáze: | OpenAIRE |
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