Fractionation of apple procyanidins according to their degree of polymerization by normal-phase high-performance liquid chromatography
| Autor: | Tomoya Takahashi, Shinkichi Honda, Akio Yanagida, Tomomasa Kanda, Takako Hamazono, Ayako Kamimura |
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| Rok vydání: | 2000 |
| Předmět: |
Chromatography
Polymers Methyl acetate Organic Chemistry Extraction (chemistry) General Medicine Fractionation Degree of polymerization Biochemistry High-performance liquid chromatography Catechin Analytical Chemistry chemistry.chemical_compound Monomer chemistry Polymerization Polyphenol Fruit Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Chromatography Gel Biflavonoids Proanthocyanidins Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 890:251-259 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(00)00614-2 |
| Popis: | A new method was developed for the fractionation of procyanidin oligomers according to their degree of polymerization. Monomeric flavan-3-ols and low molecular mass procyanidins were selectively extracted from the lyophilized powder of apple condensed tannins (ACTs) by methyl acetate extraction. Sequentially, the separation of each oligomer from dimer to pentamer in this extract was carried out by normal-phase high-performance liquid chromatography using a silica-beads packed column. The best separation was achieved with a mobile phase system containing hexane; (1) hexane-methanol-ethyl acetate, (2) hexane-acetone. These sequential treatments can be easily adapted to large-scale fractionation. |
| Databáze: | OpenAIRE |
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