Probing biofouling resistant polymer brush surfaces by atomic force microscopy based force spectroscopy

Autor: Anthony S. Clare, Nick Aldred, S.F.P. ten Donkelaar, Gyula J. Vancso, E. Kutnyanszky, Peter Manfred Schön, M. Santonicola, T. Tecim
Přispěvatelé: Materials Science and Technology of Polymers
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Poly(sulfobetaine methacrylate) (pSBMA) brushes
Polymers
Arginine-glycine-aspartic acid (RGD) adhesion peptide
Biofouling
Polymer brush
Methacrylate
Microscopy
Atomic Force
colloidal probing
atomic force microscopy (afm)
polymer brushes
arginine-glycine-aspartic acid (rgd) adhesion peptide
biofouling
atomic force microscopy
biointerfaces
protein adsorption
fibronectin
antifouling surfaces
poly(sulfobetaine methacrylate) (psbma) brushes
chemistry.chemical_compound
Colloid and Surface Chemistry
Polymer chemistry
Physical and Theoretical Chemistry
Fibronectin
Colloidal probing
Chemistry
Force spectroscopy
Surfaces and Interfaces
General Medicine
Adhesion
n/a OA procedure
Fibronectins
Chemical force microscopy
Chemical engineering
Methacrylic acid
Atomic force microscopy (AFM)
Peptides
Oligopeptides
Biotechnology
Protein adsorption
Zdroj: Colloids and surfaces B: Biointerfaces, 102, 923-930. Elsevier
ISSN: 0927-7765
DOI: 10.1016/j.colsurfb.2012.09.021
Popis: The protein repellency and biofouling resistance of zwitterionic poly(sulfobetaine methacrylate)(pSBMA) brushes grafted via surface initiated polymerization (SIP) from silicon and glass substrata was assessed using atomic force microscopy (AFM) adherence experiments. Laboratory settlement assays were conducted with cypris larvae of the barnacle Balanus amphitrite. AFM adherence includes the determination of contact rupture forces when AFM probe tips are withdrawn from the substratum. When the surface of the AFM tip is modified, adherence can be assessed with chemical specifity using a method known as chemical force microscopy (CFM). In this study, AFM tips were chemically functionalized with (a) fibronectin- here used as model for a nonspecifically adhering protein - and (b) arginine-glycine-aspartic acid (RGD) peptide motifs covalently attached to poly(methacrylic acid) (PMAA) brushes as biomimics of cellular adhesion receptors. Fibronectin functionalized tips showed significantly reduced nonspecific adhesion to pSBMA-modified substrata compared to bare gold (2.3 ± 0.75. nN) and octadecanethiol (ODT) self-assembled monolayers (1.3 ± 0.75. nN). PMAA and PMAA-RGD modified probes showed no significant adhesion to pSBMA modified silicon substrata. The results gathered through AFM protein adherence studies were complemented by laboratory fouling studies, which showed no adhesion of cypris larvae of Balanus amphitrite on pSBMA. With regard to its unusually high non-specific adsorption to a wide variety of materials the behavior of fibronectin is analogous to the barnacle cyprid temporary adhesive that also binds well to surfaces differing in polarity, charge and free energy. The antifouling efficacy of pSBMA may, therefore, be directly related to the ability of this surface to resist nonspecific protein adsorption.
Databáze: OpenAIRE
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