Sedimentation of multicomponent viruses: evaluation of sedimentation coefficient ratios
| Autor: | Ortus W. Barnett, Lyndon L. Larcom |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Glycerol
Sucrose Chromatography biology Component (thermodynamics) Sedimentation biology.organism_classification Virus Microbiology Plant Viruses Solvent Sedimentation coefficient Infectious Diseases Alfalfa mosaic virus Mosaic Viruses Virology Centrifugation Density Gradient Solvents Centrifugation Medicago sativa |
| Zdroj: | Intervirology. 9(1) |
| ISSN: | 0300-5526 |
| Popis: | Ratios of the sedimentation coefficients for alfalfa mosaic virus components are shown to be independent of the virus concentration and the density of the solvent. Different numbers of components are observed in solvents of different density. This implies that in sedimentation velocity experiments an estimate of the number of components of a multicomponent virus should involve centrifugation in solvents of different density. For some viruses, estimates of the sedimentation coefficients of individual components can be obtained from the coefficient ratios observed in unfractionated solutions and the sedimentation coefficient of the most easily purified component. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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