Baseline and 6-Week follow-up levels of PAF and activity of its metabolic enzymes in patients with heart failure and healthy volunteers--a pilot study
| Autor: | Demosthenes B. Panagiotakos, Christodoulos Stefanadis, Christos Pitsavos, Tzortzis Nomikos, Christina Chrysohoou, Smaragdi Antonopoulou, Carmen Vassiliadou, Paraskevi Detopoulou, Iason Kotroyiannis, Elizabeth Fragopoulou |
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| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Inflammation Pilot Projects Isozyme Dithiothreitol chemistry.chemical_compound Phospholipase A2 Acetyltransferases Internal medicine medicine Leukocytes Choline Humans Platelet Activating Factor Aged Heart Failure Platelet-activating factor biology business.industry Catabolism respiratory system Middle Aged medicine.disease Endocrinology chemistry Heart failure Diacylglycerol Cholinephosphotransferase biology.protein lipids (amino acids peptides and proteins) medicine.symptom Cardiology and Cardiovascular Medicine business Biomarkers Follow-Up Studies |
| Zdroj: | Angiology. 64(7) |
| ISSN: | 1940-1574 |
| Popis: | This study aimed at evaluating the changes in platelet-activating factor (PAF) and its metabolic enzymes over a 6-week follow-up period in patients with newly diagnosed heart failure ([HF] n = 12) compared with age-, sex-, and BMI-matched apparently healthy volunteers (n = 10). The PAF, its key biosynthetic enzymes (lyso-PAF acetyltransferase [lyso-PAF-AT] and dithiothreitol [DTT]-insensitive CDP choline: 1-alkyl-2-acetyl-sn-glycerol cholinephosphotransferase [PAF-CPT]), and its catabolic isoenzymes (PAF-acetylhydrolase [PAF-AH] and lipoprotein-associated phospholipase A2 [Lp-PLA2]) were measured in serum and leukocytes of participants. At baseline, patients with HF had lower median activities of lyso-PAF-AT ( P < .001) and PAF-CPT ( P = .07) in parallel with PAF levels ( P = .05) and higher activities of PAF-AH ( P = .02) and Lp-PLA2 ( P < .001) than controls. At follow-up, PAF-CPT and PAF levels marginally increased ( P = .1), lyso-PAF-AT ( P < .001) remained downregulated, while PAF-AH ( P = .004) and Lp-PLA2 ( P < .001) remained elevated compared with the controls. Newly diagnosed patients with HF under drug treatment have an affected profile of PAF biosynthetic enzymes and especially lyso-PAF-AT. |
| Databáze: | OpenAIRE |
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