Global Gene Expression in Coprinopsis cinerea Meiotic Mutants Reflects Checkpoint Arrest
| Autor: | Claire Burns, Erika Anderson, Miriam E. Zolan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
DNA Replication
Premeiotic DNA replication checkpoint arrest Investigations Coprinus Fungal Proteins 03 medical and health sciences Prophase Meiosis Gene Expression Regulation Fungal Gene expression Genetics Meiotic S phase Molecular Biology Gene MRN complex Genetics (clinical) 030304 developmental biology 0303 health sciences biology 030306 microbiology Gene Expression Profiling fungi Wild type Cell Cycle Checkpoints biology.organism_classification meiotic S phase DNA-Binding Proteins Coprinopsis cinerea mushroom development Mutation microarray analysis |
| Zdroj: | G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| Popis: | The basidiomycete Coprinopsis cinerea is well-suited to studies of meiosis because meiosis progresses synchronously in 10 million cells within each mushroom cap. Approximately 20% of C. cinerea genes exhibit changing expression during meiosis, but meiosis and mushroom development happen concurrently and therefore differentially expressed genes might not be directly involved in meiotic processes. By using microarrays, we examined global gene expression across a meiotic time course in two mutants in which meiosis arrests but mushrooms develop normally. Genes differentially expressed in the mutants compared with the wild type are likely to be involved in meiosis and sporulation as opposed to mushroom development. In rad50-1, which arrests in late prophase, RNA abundance for a group of early meiotic genes remains high, whereas the expression of a group of late meiotic genes is never induced. In contrast, in msh5-22 (which fails to undergo premeiotic DNA replication), both early and late meiotic genes are underexpressed relative to wild type at late meiotic time points as the cells die. Genes that are differentially expressed relative to wild type in both mutants are particularly strong candidates for playing roles in meiosis and sporulation. |
| Databáze: | OpenAIRE |
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