Role of B Cells in Host Defense against Primary Coxiella burnetii Infection
| Autor: | Laura Schoenlaub, Guoquan Zhang, William J. Mitchell, Alexandra Elliott, Danielle Freches |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adoptive cell transfer
Immunology B-Lymphocyte Subsets Gene Expression Spleen Mice SCID Microbiology Mice Immune system Phagocytosis Immunity Agammaglobulinaemia Tyrosine Kinase medicine Animals Pathogen Mice Knockout Mice Inbred BALB C Host Response and Inflammation biology Protein-Tyrosine Kinases bacterial infections and mycoses Coxiella burnetii biology.organism_classification Adoptive Transfer Virology Immunity Humoral Interleukin-10 Mice Inbred C57BL Interleukin 10 Infectious Diseases medicine.anatomical_structure Host-Pathogen Interactions biology.protein bacteria Female Parasitology Antibody Q Fever |
| Zdroj: | Infection and Immunity. 83:4826-4836 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.01073-15 |
| Popis: | Despite Coxiella burnetii being an obligate intracellular bacterial pathogen, our recent study demonstrated that B cells play a critical role in vaccine-induced immunity to C. burnetii infection by producing protective antibodies. However, the role of B cells in host defense against primary C. burnetii infection remains unclear. In this study, we investigated whether B cells play an important role in host defense against primary C. burnetii infection. The results showed that peritoneal B cells were able to phagocytose virulent C. burnetii bacteria and form Coxiella -containing vacuoles (CCVs) and that C. burnetii can infect and replicate in peritoneal B1a subset B cells in vitro , demonstrating a potential role for peritoneal B cells in host defense against C. burnetii infection in vivo . In addition, the results showing that B1a cells secreted a high level of interleukin-10 (IL-10) in response to C. burnetii infection in vitro suggest that B1a cells may play an important role in inhibiting the C. burnetii infection-induced inflammatory response. The observation that adoptive transfer of peritoneal B cells did not significantly affect the severity of C. burnetii infection-induced diseases in both severe combined immunity-deficient (SCID) and μMT mice indicates that peritoneal B cells alone may not be able to control C. burnetii infection. In contrast, our finding that C. burnetii infection induced more-severe splenomegaly and a higher bacterial burden in the spleens of B1a cell-deficient Bruton's tyrosine kinase x-linked immunity-deficient (BTK xid ) mice than in their wild-type counterparts further suggests that B1a cells play an important role in host defense against primary C. burnetii infection. |
| Databáze: | OpenAIRE |
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