Detection of malignant effusions: Comparison of a telomerase assay and cytologic examination
| Autor: | Pu Yan, Isabelle Guilleret, Jean Benhattar, Richard Braunschweig, Anca Mihaescu, Françoise Delacrétaz, Fred T. Bosman |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Quality Control Pathology medicine.medical_specialty Telomerase Histology Adolescent Pericardial Effusion Telomeric repeat Pathology and Forensic Medicine Malignant effusion Cytology Tumor Cells Cultured Ascitic Fluid Humans Medicine Child Aged Aged 80 and over business.industry Positive Cytology General Medicine Middle Aged Predictive value Body Fluids Pleural Effusion Malignant Cytopathology Child Preschool Cancer cell Female business |
| Zdroj: | Diagnostic Cytopathology. 24:174-180 |
| ISSN: | 1097-0339 8755-1039 |
| DOI: | 10.1002/1097-0339(200103)24:3<174::aid-dc1036>3.0.co;2-8 |
| Popis: | Telomerase is inactive in most somatic cells, but has been found to be reactivated in a majority of cancers. Our principal goal was to test whether the presence of telomerase activity concurred with positive cytology, and was thus of potential use in detecting cancer cells in effusions. The telomeric repeat amplification protocol (TRAP) assay and cytological examination were performed in a blinded fashion on 91 unselected effusions, for which laboratory processing was done according to standard procedures. In our series, 30% (27/91) of samples were found to be malignant by cytology. Of these, 19 (70%) were also positive in the TRAP assay. Of the 8 telomerase-negative cytology-positive samples, RNA integrity was generally poor, indicating suboptimal sample conservation for molecular analysis. Negative cytology in the presence of telomerase activity was observed in 17 effusions. Of these, 11 were from patients with advanced cancer, and thus a diagnosis of malignant effusion should be suspected. The TRAP assay for telomerase activity holds promise in the analysis of effusions, but its routine use as an adjunct to cytology awaits further confirmation of its positive predictive value. Diagn. Cytopathol. 2001;24:174–180. © 2001 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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