Adherent/invasive Escherichia coli (AIEC) isolates from asymptomatic people: new E. coli ST131 O25:H4/H30-Rx virotypes
| Autor: | Patricia Lozano-Zarain, Claudia F. Martinez de la Peña, Carmen Torres, Rosa del Carmen Rocha-Gracia, Margarita M. P. Arenas-Hernández, Ygnacio Martínez-Laguna, Gerardo Cortés-Cortés, Edwin Barrios-Villa |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Serotype Crohn’s disease 030106 microbiology IBD lcsh:QR1-502 Virulence Drug resistance Biology medicine.disease_cause Virotype Serogroup lcsh:Microbiology Bacterial Adhesion lcsh:Infectious and parasitic diseases Microbiology 03 medical and health sciences Feces medicine Humans Uropathogenic Escherichia coli lcsh:RC109-216 Escherichia coli Gene Research Escherichia coli Proteins lcsh:RM1-950 General Medicine Pathogenicity island Multiple drug resistance lcsh:Therapeutics. Pharmacology Infectious Diseases Parasitology Asymptomatic Diseases AIEC |
| Zdroj: | Annals of Clinical Microbiology and Antimicrobials Annals of Clinical Microbiology and Antimicrobials, Vol 17, Iss 1, Pp 1-11 (2018) RIUR. Repositorio Institucional de la Universidad de La Rioja instname |
| ISSN: | 1476-0711 |
| Popis: | Background The widespread Escherichia coli clone ST131 implicated in multidrug-resistant infections has been recently reported, the majority belonging to O25:H4 serotype and classified into five main virotypes in accordance with the virulence genes carried. Methods Pathogenicity Islands I and II (PAI-I and PAI-II) were determined using conventional PCR protocols from a set of four E. coli CTXR ST131 O25:H4/H30-Rx strains collected from healthy donors’ stool. The virulence genes patterns were also analyzed and compared them with the virotypes reported previously; then adherence, invasion, macrophage survival and biofilm formation assays were evaluated and AIEC pathotype genetic determinants were investigated. Findings Non-reported virulence patterns were found in our isolates, two of them carried satA, papA, papGII genes and the two-remaining isolates carried cnfI, iroN, satA, papA, papGII genes, and none of them belonged to classical ST131 virotypes, suggesting an endemic distribution of virulence genes and two new virotypes. The presence of PAI-I and PAI-II of Uropathogenic E. coli was determined in three of the four strains, furthermore adherence and invasion assays demonstrated higher degrees of attachment/invasion compared with the control strains. We also amplified intI1, insA and insB genes in all four samples. Interpretation The results indicate that these strains own non-reported virotypes suggesting endemic distribution of virulence genes, our four strains also belong to an AIEC pathotype, being this the first report of AIEC in México and the association of AIEC with healthy donors. |
| Databáze: | OpenAIRE |
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