Molecular eco-epidemiology on the sympatric Chagas disease vectors Triatoma brasiliensis and Triatoma petrocchiae: Ecotopes, genetic variation, natural infection prevalence by trypanosomatids and parasite genotyping
| Autor: | Jane Costa, Tarcianne M Lima-Oliveira, Myriam Harry, Fernanda von Hertwig Mascarenhas Fontes, Carlos Eduardo Almeida, Carolina Valença-Barbosa, Maurício Lilioso, Elaine Folly-Ramos, Dayane Pires-Silva, Jonathan Filée, Jose Gabriel Vergara Meza, Marta Maria Geraldes Teixeira |
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| Přispěvatelé: | Evolution, génomes, comportement et écologie (EGCE), Institut de Recherche pour le Développement (IRD)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Universidade Federal da Paraiba (UFPB) |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Chagas disease Species complex Genotype Range (biology) Trypanosoma cruzi Veterinary (miscellaneous) 030231 tropical medicine Zoology 03 medical and health sciences 0302 clinical medicine parasitic diseases Prevalence medicine Animals Chagas Disease Triatoma Ecosystem Molecular Epidemiology Genetic diversity biology [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] Genetic Variation 030108 mycology & parasitology biology.organism_classification medicine.disease Triatoma brasiliensis Insect Vectors 3. Good health Sympatry Infectious Diseases Insect Science Vector (epidemiology) Parasitology Brazil |
| Zdroj: | Acta Tropica Acta Tropica, Elsevier, 2020, 201, pp.105188. ⟨10.1016/j.actatropica.2019.105188⟩ Acta Tropica, 2020, 201, pp.105188. ⟨10.1016/j.actatropica.2019.105188⟩ |
| ISSN: | 0001-706X |
| Popis: | International audience; Triatoma petrocchiae is the newly member of the Triatoma brasiliensis species complex. This species overlaps with T. brasiliensis in geographic and ecotypic occupation in the sylvatic habitat because both inhabit rocky outcrops in the semi-arid portion of Brazilian northeast. In this region T. brasiliensis is the most important Chagas disease vector because it constantly colonizes domiciles. In contrast, T. petrocchiae is rarely found in peri or intradomiciliary habitats-reason why little is known about this species. Therefore, Here, we present information for the first time on. the T. petrocchiae ecotopes, genetic diversity, Trypanosoma cruzi prevalence/genotyping in comparison to T. brasiliensis. We found T. brasilensis (N = 223) and T. petrocchiae (N = 69) in co-habitation in rocky outcrops in three Districts of Paraíba and Rio Grande do Norte states. Forty-tree T. petrocchiae insects of eleven sampling spots (composing three geographic populations) were genotyped for the mitochondrial Cyt B gene and little geographic structure was observed. Tajima's D test suggested that species is evolving toward a mutation-drift equilibrium in our collection range. Sylvatic T. petrocchiae had 4% (3/68) of infected insects by T. cruzi, whereas T. brasiliensis had 26% (59/223). Fluorescent Fragment Length Barcoding demonstrated that all three T. petrocchiae harbored TcI whereas T. brasiliensis had TcI, but also TcIII, TcII/TcVI and T. rangeli genotype A, sometimes under mixed infections. None of infected T. petrocchiae were carrying mixed infections. However, this result should be confirmed using a larger pool of infected bugs. We here presented the first documentation of T. rangeli infecting T. brasiliensis. The finding of infected T. petrocchiae calls for constant vector monitoring because the epidemiologic scenario is dynamic and sylvatic vectors are progressively found in adaptation to anthropic environments. |
| Databáze: | OpenAIRE |
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