Metalloprotease-Dependent Onset of Blood Circulation in Zebrafish
| Autor: | Daigo Nishimura, Atsuko Sehara-Fujisawa, Miki Takeuchi, Atsuo Iida, Kazuyo Misaki, Kazuya Sakaguchi, Hidetoshi Sakurai, Kiyoaki Sato, Atsuo Kawahara, Shigenobu Yonemura, Aya Iwaki, Makoto Kobayashi |
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| Jazyk: | angličtina |
| Předmět: |
Metalloproteinase
Agricultural and Biological Sciences(all) biology Biochemistry Genetics and Molecular Biology(all) Lumen (anatomy) Embryo DEVBIO biology.organism_classification General Biochemistry Genetics and Molecular Biology Cell biology Plasma flow Blood circulation Blood Circulation Immunology Circulatory system Metalloproteases Animals CELLBIO General Agricultural and Biological Sciences Zebrafish ADAM8 |
| Zdroj: | Current Biology. (12):1110-1116 |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/j.cub.2010.04.052 |
| Popis: | Summary The primitive blood circulation requires intravascular plasma flow [1]. However, it remains unclear whether the onset of earliest blood circulation is dependent solely on establishment of a functional circulatory organ or whether it also requires active processes inherent in blood cells. In this study, we present novel mechanisms for the onset of blood circulation by monitoring fluorescently labeled blood precursors and blood vessels in zebrafish. The earliest blood circulation occurs synchronously. This synchrony is achieved by the retention of erythroid precursors on the lumen of the vasculature after their invasion from the subaortic region, and then by simultaneous release of these precursors into the flow. Morphological and biochemical analyses suggest that the onset of blood circulation accompanies disruption of blood cell-to-vessel adhesion and requires metalloprotease-dependent processes. ADAM8, a member of the a d isintegrin a nd m etalloprotease (ADAM) family, mediates the onset of blood circulation. In ADAM8-depleted embryos, erythroid cells fail to detach from the vascular lumen and stagnate. Expression of a protease-defective ADAM8 in erythroid cells causes dominant-negative effects on blood circulation, suggesting cell-autonomous roles of ADAM8. Based on these findings, we propose that the first erythroid cells require both flow-dependent passive and proteolysis-dependent active processes to enter the circulation. |
| Databáze: | OpenAIRE |
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