Crystallization and preliminary crystallographic analysis of endo-1,3-β-glucanase from Arthrobacter sp
| Autor: | Masatake Ohnishi, You-Na Kang, Masayuki Oda, Zhongcun Pang, Ryo Kobayashi, Bunzo Mikami, Mizuho Ban |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Models
Molecular Glycoside Hydrolases Stereochemistry Biophysics Crystallography X-Ray Biochemistry Protein Structure Secondary law.invention Hydrolysis Bacterial Proteins Structural Biology law Arthrobacter Genetics Crystallization biology Chemistry Resolution (electron density) Space group Glucanase Condensed Matter Physics biology.organism_classification Chromatography Ion Exchange Solvent Crystallography Crystallization Communications X-ray crystallography Chromatography Gel |
| Popis: | Endo-1,3-beta-glucanases hydrolyze internal 1,3-beta-glucosyl linkages. The endo-1,3-beta-glucanase from Arthrobacter sp. was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group P4(1), with unit-cell parameters a = 71.31, c = 60.07 A, and contained one molecule per asymmetric unit. The Matthews coefficient (VM) and the solvent content were 2.35 A3 Da(-1) and 47.63%, respectively. Diffraction data were collected to a resolution of 1.66 A at SPring-8 using a MAR CCD area detector and gave a data set with an overall Rmerge of 5.4% and a completeness of 99.4%. |
| Databáze: | OpenAIRE |
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