Mutagenic Potential of Bos taurus Papillomavirus Type 1 E6 Recombinant Protein: First Description
| Autor: | Roberta Fiusa Magnelli, Edislane Barreiros de Souza, R.C. Stocco, Márcio Augusto Caldas Rocha de Carvalho, Rodrigo Pinheiro Araldi, J. Mazzuchelli-de-Souza, Paulo Luis de Sá Júnior, R.F. Carvalho, Thatiana Correa de Melo, Willy Beçak, Diva Denelle Spadacci-Morena, Diego Grando Módolo |
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| Přispěvatelé: | Instituto Butantan, Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Genome instability
Article Subject Carcinogenesis DNA damage viruses lcsh:Medicine medicine.disease_cause Genomic Instability General Biochemistry Genetics and Molecular Biology Cell Line law.invention law medicine Animals Humans Papillomaviridae Cyclophosphamide Bovine papillomavirus 1 Bovine papillomavirus General Immunology and Microbiology biology lcsh:R Papillomavirus Infections Oncogene Proteins Viral General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Virology Molecular biology Recombinant Proteins Comet assay Cell culture Recombinant DNA Cattle Research Article |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP BioMed Research International BioMed Research International, Vol 2015 (2015) |
| Popis: | Made available in DSpace on 2018-12-11T17:26:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-01-01 Bovine papillomavirus (BPV) is considered a useful model to study HPV oncogenic process. BPV interacts with the host chromatin, resulting in DNA damage, which is attributed to E5, E6, and E7 viral oncoproteins activity. However, the oncogenic mechanisms of BPV E6 oncoprotein per se remain unknown. This study aimed to evaluate the mutagenic potential of Bos taurus papillomavirus type 1 (BPV-1) E6 recombinant oncoprotein by the cytokinesis-block micronucleus assay (CBMNA) and comet assay (CA). Peripheral blood samples of five calves were collected. Samples were subjected to molecular diagnosis, which did not reveal presence of BPV sequences. Samples were treated with 1 g/mL of BPV-1 E6 oncoprotein and 50 g/mL of cyclophosphamide (positive control). Negative controls were not submitted to any treatment. The samples were submitted to the CBMNA and CA. The results showed that BPV E6 oncoprotein induces clastogenesis per se, which is indicative of genomic instability. These results allowed better understanding the mechanism of cancer promotion associated with the BPV E6 oncoprotein and revealed that this oncoprotein can induce carcinogenesis per se. E6 recombinant oncoprotein has been suggested as a possible vaccine candidate. Results pointed out that BPV E6 recombinant oncoprotein modifications are required to use it as vaccine. Laboratório de Genética Instituto Butantan Programa de Pós-Graduação Interunidades em Biotecnologia Instituto de Ciências Biomédicas (ICB) Universidade de São Paulo (USP) Laboratório de Biologia Molecular Genética e Mutagênese Departamento de Biologia Faculdade de Ciências e Letras de Assis (FCLA) Universidade Estadual Paulista Júlio de Mesquista Filho (UNESP) Laboratório de Fisiopatologia Instituto Butantan Laboratório de Biologia Molecular Genética e Mutagênese Departamento de Biologia Faculdade de Ciências e Letras de Assis (FCLA) Universidade Estadual Paulista Júlio de Mesquista Filho (UNESP) |
| Databáze: | OpenAIRE |
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