Comparative Proteomic Analysis of Molecular Differences between Leaves of Wild-Type Upland Cotton and Its Fuzzless-Lintless Mutant
| Autor: | Xiang Jin, Chengcheng Tao, Bowen Zheng, Wangyang Song, Hongbin Li, Liping Zhu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Chlorophyll b Mutant fuzzless-lintless mutant Pharmaceutical Science Biology Secondary metabolite 01 natural sciences Article Analytical Chemistry lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound proteomics lcsh:Organic chemistry Gene Expression Regulation Plant Drug Discovery medicine Gene Regulatory Networks chlorophyll Protein Interaction Maps Physical and Theoretical Chemistry Secondary metabolism 030304 developmental biology Plant Proteins 0303 health sciences Gossypium Gene Expression Profiling Organic Chemistry Wild type gossypol Plant Leaves chemistry Biochemistry Chemistry (miscellaneous) Gossypol Chlorophyll Proteome Mutation Molecular Medicine Energy Metabolism cotton leaves 010606 plant biology & botany medicine.drug |
| Zdroj: | Molecules, Vol 24, Iss 20, p 3769 (2019) Molecules Volume 24 Issue 20 |
| ISSN: | 1420-3049 |
| Popis: | Fuzzless-lintless mutant (fl) ovules of upland cotton have been used to investigate cotton fiber development for decades. However, the molecular differences of green tissues between fl and wild-type (WT) cotton were barely reported. Here, we found that gossypol content, the most important secondary metabolite of cotton leaves, was higher in Gossypium hirsutum L. cv Xuzhou-142 (Xu142) WT than in fl. Then, we performed comparative proteomic analysis of the leaves from Xu142 WT and its fl. A total of 4506 proteins were identified, of which 103 and 164 appeared to be WT- and fl-specific, respectively. In the 4239 common-expressed proteins, 80 and 74 were preferentially accumulated in WT and fl, respectively. Pathway enrichment analysis and protein&ndash protein interaction network analysis of both variety-specific and differential abundant proteins showed that secondary metabolism and chloroplast-related pathways were significantly enriched. Quantitative real-time PCR confirmed that the expression levels of 12 out of 16 selected genes from representative pathways were consistent with their protein accumulation patterns. Further analyses showed that the content of chlorophyll a in WT, but not chlorophyll b, was significantly increased compared to fl. This work provides the leaf proteome profiles of Xu142 and its fl mutant, indicating the necessity of further investigation of molecular differences between WT and fl leaves. |
| Databáze: | OpenAIRE |
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