NINJA connects the co-repressor TOPLESS to jasmonate signalling
| Autor: | Jose Manuel Chico, Amparo Cuéllar Pérez, Geert De Jaeger, Gemma Fernández Barbero, Wim Grunewald, Alain Goossens, Jared A. Sewell, Robin Vanden Bossche, Erwin Witters, Eduardo Gil, Roberto Solano, Jan Geerinck, Jeff A. Long, Sofie Tilleman, Gloria García-Casado, Dirk Inzé, Laurens Pauwels |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Protein family
Arabidopsis Repressor Cyclopentanes Models Biological Article Transactivation Gene Expression Regulation Plant Two-Hybrid System Techniques Jasmonate Oxylipins Biology Regulation of gene expression Genetics Multidisciplinary biology Arabidopsis Proteins Gene Expression Profiling Signal transducing adaptor protein biology.organism_classification Plants Genetically Modified Repressor Proteins Functional genomics Protein Binding Signal Transduction |
| Zdroj: | Nature |
| ISSN: | 1476-4687 0028-0836 |
| Popis: | Jasmonoyl-isoleucine (JA-Ile) is a plant hormone that regulates a broad array of plant defence and developmental processes1, 2, 3, 4, 5. JA-Ile-responsive gene expression is regulated by the transcriptional activator MYC2 that interacts physically with the jasmonate ZIM-domain (JAZ) repressor proteins. On perception of JA-Ile, JAZ proteins are degraded and JA-Ile-dependent gene expression is activated6, 7. The molecular mechanisms by which JAZ proteins repress gene expression remain unknown. Here we show that the Arabidopsis JAZ proteins recruit the Groucho/Tup1-type co-repressor TOPLESS (TPL)8 and TPL-related proteins (TPRs) through a previously uncharacterized adaptor protein, designated Novel Interactor of JAZ (NINJA). NINJA acts as a transcriptional repressor whose activity is mediated by a functional TPL-binding EAR repression motif. Accordingly, both NINJA and TPL proteins function as negative regulators of jasmonate responses. Our results point to TPL proteins as general co-repressors that affect multiple signalling pathways through the interaction with specific adaptor proteins. This new insight reveals how stress-related and growth-related signalling cascades use common molecular mechanisms to regulate gene expression in plants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|