Cloning of the hamster p16 gene 5' upstream region and its aberrant methylation patterns in pancreatic cancer
| Autor: | Hiromasa Fujii, Mayumi Shigemura, Kyoko Shimizu, Toshifumi Tsujiuchi, Ayumi Kato, Kanya Honoki, Madoka Hanaoka |
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| Rok vydání: | 2005 |
| Předmět: |
Male
5' Flanking Region Bisulfite sequencing Biophysics Hamster Biology Biochemistry Polymerase Chain Reaction Pancreatic cancer Cricetinae medicine Animals Humans Epigenetics Cloning Molecular Promoter Regions Genetic Molecular Biology Gene Cyclin-Dependent Kinase Inhibitor p16 Pancreatic duct Chromosome Aberrations Mesocricetus Genes p16 Cell Biology Methylation Sequence Analysis DNA DNA Methylation medicine.disease Molecular biology Pancreatic Neoplasms medicine.anatomical_structure DNA methylation Female |
| Zdroj: | Biochemical and biophysical research communications. 333(4) |
| ISSN: | 0006-291X |
| Popis: | The hamster model of pancreatic carcinogenesis is useful for understanding the development of human pancreatic cancer. However, there is only a small amount of hamster genetic information available for analyzing the gene alterations in hamster pancreatic cancers. Here, we determined the nucleotide sequence of the 5′ upstream region of the hamster p16 gene using a suppression polymerase chain reaction method combined with gene-specific primers. Based on this sequence, we analyzed the methylation status of the 5′ region by bisulfite sequencing in three normal pancreatic tissues and five pancreatic duct adenocarcinomas (PDAs). All five PDAs were highly methylated in the 5′ upstream region and showed reduced expressions of the p16 gene, while the three normal samples were demethylated. The method described in this study is a highly effective and rapid technique for determining the 5′ upstream region, and is applicable to epigenetic studies of the methylation status of this region. |
| Databáze: | OpenAIRE |
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