Plant Thaumatin-like Proteins: Function, Evolution and Biotechnological Applications
| Autor: | João Pacífico Bezerra-Neto, Ilza Maria Sittolin, Francisco R. Freire-Filho, Valesca Pandolfi, José Ribamar Costa Ferreira-Neto, Genira Pereira-Andrade, Carolline de Jesús-Pires, Eliseu Binneck, Ana Carolina Wanderley-Nogueira, Ana Maria Benko-Iseppon, Antônio Félix da Costa, Ederson Akio Kido, Roberta Lane de Oliveira Silva, Gilvan Pio-Ribeiro |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antifungal Agents Protein family Antineoplastic Agents Computational biology Biology Plant Roots 01 natural sciences Biochemistry Transcriptome 03 medical and health sciences Gene Expression Regulation Plant Osmotic Pressure Stress Physiological Molecular Biology Gene Phylogeny Plant Proteins Abiotic component Dehydration Abiotic stress Vigna fungi Computational Biology food and beverages Cell Biology General Medicine Biotic stress Recombinant Proteins Droughts Flavoring Agents Plant Leaves Plant Breeding 030104 developmental biology Thaumatin Multigene Family Function (biology) 010606 plant biology & botany |
| Zdroj: | Current Protein & Peptide Science. 21:36-51 |
| ISSN: | 1389-2037 |
| DOI: | 10.2174/1389203720666190318164905 |
| Popis: | Thaumatin-like proteins (TLPs) are a highly complex protein family associated with host defense and developmental processes in plants, animals, and fungi. They are highly diverse in angiosperms, for which they are classified as the PR-5 (Pathogenesis-Related-5) protein family. In plants, TLPs have a variety of properties associated with their structural diversity. They are mostly associated with responses to biotic stresses, in addition to some predicted activities under drought and osmotic stresses. The present review covers aspects related to the structure, evolution, gene expression, and biotechnological potential of TLPs. The efficiency of the discovery of new TLPs is below its potential, considering the availability of omics data. Furthermore, we present an exemplary bioinformatics annotation procedure that was applied to cowpea (Vigna unguiculata) transcriptome, including libraries of two tissues (root and leaf), and two stress types (biotic/abiotic) generated using different sequencing approaches. Even without using genomic sequences, the pipeline uncovered 56 TLP candidates in both tissues and stresses. Interestingly, abiotic stress (root dehydration) was associated with a high number of modulated TLP isoforms. The nomenclature used so far for TLPs was also evaluated, considering TLP structure and possible functions identified to date. It is clear that plant TLPs are promising candidates for breeding purposes and for plant transformation aiming a better performance under biotic and abiotic stresses. The development of new therapeutic drugs against human fungal pathogens also deserves attention. Despite that, applications derived from TLP molecules are still below their potential, as it is evident in our review. |
| Databáze: | OpenAIRE |
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