Lack of interference of common phytoecdysteroids with production of nitric oxide by immune-activated mammalian macrophages
Autor: | Eva Kmoníčková, Zdeněk Zídek, Juraj Harmatha, Karel Vokáč |
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Rok vydání: | 2008 |
Předmět: |
Lipopolysaccharides
Lipopolysaccharide Clinical Biochemistry 20-Hydroxyecdysone Phytoecdysteroid Biology Nitric Oxide Biochemistry Nitric oxide Interferon-gamma Mice chemistry.chemical_compound Endocrinology Immune system Animals Molecular Biology Cells Cultured Pharmacology chemistry.chemical_classification Ecdysteroid Molecular Structure Macrophages Organic Chemistry Ecdysteroids Macrophage Activation In vitro Mice Inbred C57BL chemistry Female Corticosterone Lactone |
Zdroj: | Steroids. 73:466-471 |
ISSN: | 0039-128X |
Popis: | Effects of selected common phytoecdysteroids on immunobiological responses triggered by lipopolysaccharide and interferon-γ (IFN-γ) were tested under in vitro conditions using murine resident peritoneal macrophages. Namely, production of nitric oxide was investigated. The series of test agents encompassed ecdysteroids occurring often as major components of the ecdysteroid fraction in numerous plant extracts: 20-hydroxyecdysone (20E), polypodine B, ajugasterone C, ponasterone A and inokosterone. Their structural variability concerns only variation in the number and position of hydroxyls. Two additional side-chain modified ecdysteroids: makisterone A (with a methyl substituent at position 24) and carthamosterone (with a cyclic side-chain lactone), and three ecdysteroid analogs: poststerone, rubrosterone and dihydrorubrosterone (devoid of side chains) were included into the test series. All test compounds, except of ponasterone A, represent natural substances isolated from the medicinal plant Leuzea carthamoides and are supposed to be significant for the often reported pharmacological activities of preparations derived from this species. However, the tested ecdysteroids did not interfere with the immunobiological activity of the immunocompetent cells. Our results thus differ from the so far reported information. |
Databáze: | OpenAIRE |
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