Mesenchymal stem cells maintain their defining stem cell characteristics after treatment with cisplatin
| Autor: | Alexander Rühle, Jürgen Debus, Nils H. Nicolay, Peter E. Huber, Ramon Lopez Perez, Sonevisay Sisombath, Rainer Saffrich, Klaus J. Weber, Anthony D. Ho, Thuy Trinh |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cellular differentiation Drug Resistance Apoptosis Bone Marrow Cells Biology Bioinformatics Article 03 medical and health sciences 0302 clinical medicine Heat shock protein medicine Humans Regeneration Cells Cultured Stem cell transplantation for articular cartilage repair Cell Proliferation Cisplatin Multidisciplinary Cell growth Regeneration (biology) Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Fibroblasts 030104 developmental biology Phenotype 030220 oncology & carcinogenesis Cancer research Stem cell medicine.drug |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep20035 |
| Popis: | Mesenchymal stem cells (MSCs) aid the regeneration of tissues damaged by treatment with cisplatin. However, the effects of this cytotoxic drug on the stem cells have been largely unknown. Here we demonstrate that human bone marrow-derived MSCs are relatively resistant to cisplatin treatment and show resistance levels comparable to these of differentiated fibroblasts. Cisplatin did not affect cellular morphology, adhesion or induction of apoptosis in MSCs. The potential for differentiation was preserved after exposure to cisplatin and established MSC surface markers were observed to be stably expressed irrespective of cisplatin treatment. Cytoskeletal rearrangements and high expression levels of individual heat shock proteins were detected in MSCs and may be partly responsible for the observed cisplatin resistance. The cisplatin-resistant phenotype of human MSCs supports the concept of further investigating these stem cells as a potential treatment option for cisplatin-induced tissue damage. |
| Databáze: | OpenAIRE |
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