Contribution of Anaphase B to Chromosome Separation in Higher Plant Cells Estimated by Image Processing
| Autor: | Natsumaro Kutsuna, Fumi Kumagai-Sano, Seiichiro Hasezawa, Toshio Sano, Tomomi Hayashi |
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| Rok vydání: | 2007 |
| Předmět: |
DNA Replication
Physiology fungi Cell Biology Plant Science General Medicine Biology Plant cell Chromosomes Plant Spindle elongation Cell Line Green fluorescent protein Cell biology Chromosome segregation Spindle checkpoint Tubulin Tobacco Image Processing Computer-Assisted biology.protein Anaphase Chromosome separation |
| Zdroj: | Plant and Cell Physiology. 48:1509-1513 |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcm117 |
| Popis: | Anaphase can be categorized into the two subphases of anaphase A and B, but anaphase B has not been clearly described in higher plant cells. In this study, we time-sequentially followed the dynamics of chromosome segregation and spindle elongation in tobacco BY-2 cells using histone-red fluorescent protein (RFP) and green fluorescent protein (GFP)-tubulin, respectively. Construction of kymographs and determination of the positions of chromosomes and spindle edges by image processing revealed that anaphase B contributed to about 40% of the chromosome separation in distance, which is comparable with that in animal cells. These results suggest that higher plant cells potentially possess the process of anaphase B. |
| Databáze: | OpenAIRE |
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