Neorhizobium tomejilense sp. nov., first non-symbiotic Neorhizobium species isolated from a dryland agricultural soil in southern Spain
| Autor: | Margarita Gomila, Juan Imperial, Amalia Soenens |
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| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial food.ingredient Lineage (evolution) Biology Non-symbiotic Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences food Rhizobiaceae Genus RNA Ribosomal 16S Operon Botany MALDI-TOF MS Soil Microbiology Ecology Evolution Behavior and Systematics 030304 developmental biology ANIb 0303 health sciences Neorhizobium non-symbiotic phylogenetic analysis Phylogenetic analysis Phylogenetic tree 030306 microbiology Fatty Acids Dendrogram Agriculture Sequence Analysis DNA 16S ribosomal RNA rpoB Bacterial Typing Techniques Genes Bacterial Spain RRNA Operon |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | We describe for the first time a non-symbiotic species of the recently described genus Neorhizobium, lacking nodulation or nitrogen fixation genes. The strains were isolated from a dryland agricultural soil in Southern Spain where no record of legume cultivation is available, thus we propose the name Neorhizobium tomejilense sp. nov. (type strain T17_20T, LMG 30623T and CECT 9621T). N. tomejilense exhibit a clear distinct lineage from the other Neorhizobium species, N. galegae HAMBI 540T, N. alkalisoli DSM 21826T, N. huautlense DSM 21817T, based on polyphasic evidence. Phylogenetic marker analysis of 16S rDNA, atpD, glnII, recA, rpoB and thrC genes and genomic identity data derived from the draft genomic sequences showed that N. tomejilense strains clearly separated from the other Neorhizobium species and that N. galegae HAMBI 540T represents the closest type species to N. tomejilense with 90.1 % Average Nucleotide Identity (ANIb), well below the 95-96 % species circumscription threshold. Phenotypic characterisation of N. tomejilense also displayed differences with the other Neorhizobium species. Whole-cell matrix-assisted laser-desorption time-of-flight mass spectrometry (WC MALDI-TOF-MS) fingerprint analysis and the dendrogram derived from the fingerprint profiles, showed a clearly distinct group formed by the three N. tomejilense isolates (T17_20T, T20_22 and T11_12) from the other Neorhizobium especies. We thank Francisco Temprano, Dulce Rodríguez-Navarro, and Francisco Perea, from IFAPA “Las Torres-Tomejil,” for generously facilitating our soil sampling in the Tomejil experimental farm. We are grateful to Aidan Parte for expert advice on bacterial nomenclature. |
| Databáze: | OpenAIRE |
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