Changes in the motility of B16F10 melanoma cells induced by alterations in resting calcium influx
| Autor: | James L. Cox, Lancaster T, Carlson Cg |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Cancer Research
Cell Integrin Motility Gadolinium Dermatology Divalent Chlorides Cell Movement medicine Extracellular Cell Adhesion Tumor Cells Cultured Humans Calcium Signaling Terbium Melanoma chemistry.chemical_classification Ion Transport biology Chemistry Ionomycin Cobalt Calcium Channel Blockers Cell biology medicine.anatomical_structure Oncology Manganese Compounds Permeability (electromagnetism) biology.protein Biophysics Potassium Calcium Fibroblast Growth Factor 2 Steady state (chemistry) B16f10 melanoma Extracellular Space |
| Zdroj: | Melanoma research. 12(3) |
| ISSN: | 0960-8931 |
| Popis: | Alterations in the extracellular Ca(2+) or K(+) concentration had significant influences on the motility of B16F10 melanoma cells measured in the absence of exogenous integrins using a conventional Boyden chamber assay. At normal K(+) concentrations, motility increased slightly when the concentration of Ca(2+) was increased 10-fold. At normal Ca(2+) concentrations, motility increased by 290% when the extracellular K(+) concentration was reduced 10-fold (from control of 5.4 mM to 0.54 mM), and increased to 250% of control levels when the K(+) concentration was increased between 30 and 54 mM, but was relatively uninfluenced at K(+) concentrations between 5 and 30 mM. Simultaneous application of low concentrations (20 microM) of GdCl(3) completely prevented the effects of low and high K(+) on motility. Exposure to Gd(3+) or Tb(3+) also produced a flattening of the cells and enhanced cell attachment. Although the steady state intracellular Ca(2+) concentration was not significantly influenced by the K(+) concentration, the resting permeability to divalent cations, determined from Mn(2+) quench rates in fura-loaded cells, was significantly increased by a reduction in the K(+) concentration. These results indicate that resting Ca(2+) influx is critical to the movement of B16F10 melanoma cells, and demonstrate that lanthanides, which block resting Ca(2+) influx pathways, are potent antimotility agents. |
| Databáze: | OpenAIRE |
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