FcUni-RLuc: an engineered Renilla luciferase with Fc binding ability and light emission activity

Autor:	Sayyed Hamid Zarkesh-Esfahani, Rahman Emamzadeh, Mahboobeh Nazari, R. Roghanian, A. Farzannia
Rok vydání:	2015
Předmět:	Receptor ErbB-2 Breast Neoplasms Plasma protein binding Antibodies Monoclonal Humanized Protein Engineering Biochemistry Analytical Chemistry Labelling Tumor Cells Cultured Electrochemistry Humans Environmental Chemistry Bioluminescence Receptor Spectroscopy Luciferases Renilla biology Chemistry Protein engineering Trastuzumab Molecular biology Immunoglobulin Fc Fragments Molecular Probes Luminescent Measurements biology.protein Biological Assay Female Light emission Antibody Molecular probe Protein Binding Signal Transduction
Zdroj:	The Analyst. 140:1438-1441
ISSN:	1364-5528 0003-2654
DOI:	10.1039/c4an01946f
Popis:	A novel and advanced Fc-binding probe – FcUni-RLuc namely – has been produced and functionally assayed for labelling IgGs. The Fc antibody binding sequence – HWRGWV – was fused to Renilla luciferase, and the purified probe was employed for bioluminescence enzyme-linked immunoabsorbance assay of Her2 positive cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::283b6dbae614d7ae9993450b3c2ef245 https://doi.org/10.1039/c4an01946f Zobrazit plný text záznamu