Digoxin reduces the mutagenic effects of Mitomycin C in human and rodent cell lines
| Autor: | Maria Cristina Da Silva Barbosa, Juliana Pereira Lyon, Fernando de Pilla Varotti, Leonardo Marmo Moreira, Luciana Madeira da Silva, Isabella Viana Gomes da Silva, Luiz Fernando de Camargos, Vanessa J. S. V. Santos, Fábio Vieira dos Santos, Júlia Teixeira de Oliveira |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
endocrine system Clinical Biochemistry Biomedical Engineering Bioengineering Pharmacology medicine.disease_cause Ames test HeLa 03 medical and health sciences medicine Cytotoxicity biology Chemistry Mitomycin C digestive oral and skin physiology food and beverages Cell Biology biology.organism_classification Comet assay 030104 developmental biology Micronucleus test Chemoprotective Original Article Genotoxicity Biotechnology |
| Popis: | Digoxin is a drug widely used to treat heart failure and studies have demonstrated its potential as anticancer agent. In addition, digoxin presents the potential to interact with a series of other compounds used in medicine. The aim of the present study was to evaluate in vitro the cytotoxicity, genotoxicity and mutagenicity of digoxin and its potential to interact with the mutagen Mitomycin C (MMC). The cytotoxicity of digoxin was assessed by employing the MTT method and the comet assay was performed to assess the genotoxicity of this medicine in CHO-K1 and HeLa cell lines. Besides, the cytokinesis-block micronucleus assay was performed to assess the mutagenicity and the antimutagenicity of this drug. The Ames assay was also performed with TA98 and TA100 strains of S. typhimurium. Results showed that digoxin was cytotoxic, genotoxic and mutagenic for HeLa and CHO-K1 cell lines at concentrations many times higher than those observed in human therapeutic conditions. Nevertheless, an antimutagenic effect against the mutagen MMC was observed on both cell lines in concentrations near those used therapeutically in humans. This chemoprotective effect observed is an interesting finding that should be better explored regarding its impact in anticancer chemotherapy. |
| Databáze: | OpenAIRE |
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