Simultaneous determination of piperaquine and its N -oxidated metabolite in rat plasma using LC-MS/MS
| Autor: | Jie Xing, Meitong Zang, Jianbo Ji, Aijuan Yang, Huixiang Liu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Spectrometry Mass Electrospray Ionization Electrospray Formic acid 030106 microbiology Clinical Biochemistry Mass spectrometry 030226 pharmacology & pharmacy Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Drug Stability Limit of Detection Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Drug Discovery Animals Protein precipitation Sample preparation Rats Wistar Molecular Biology Pharmacology Detection limit Chromatography Selected reaction monitoring Reproducibility of Results General Medicine Rats chemistry Linear Models Quinolines Chromatography Liquid |
| Zdroj: | Biomedical Chromatography. 31:e3974 |
| ISSN: | 0269-3879 |
| DOI: | 10.1002/bmc.3974 |
| Popis: | A sensitive and efficient liquid chromatography tandem mass spectrometry method was developed and validated for the simultaneous determination of piperaquine (PQ) and its N-oxidated metabolite (PQ-M) in plasma. A simple protein precipitation procedure was used for sample preparation. Adequate chromatographic retention was achieved on a C18 column under gradient elution with acetonitrile and 2 mm aqueous ammonium acetate containing 0.15% formic acid and 0.05% trifluoroacetic acid. A triple-quadrupole mass spectrometer equipped with an electrospray source was set up in the positive ion mode and multiple reaction monitoring mode. The method was linear in the range of 2.0-400.0 ng/mL for PQ and 1.0-50.0 ng/mL for PQ-M with suitable accuracy, precision and extraction recovery. The lower limits of detection (LLOD) were established at 0.4 and 0.2 ng/mL for PQ and PQ-M, respectively, using 40 μL of plasma sample. The matrix effect was negligible under the current conditions. No effect was found for co-administrated artemisinin drugs or hemolysis on the quantification of PQ and PQ-M. Stability testing showed that two analytes remained stable under all relevant analytical conditions. The validated method was successfully applied to a pharmacokinetic study performed in rats after a single oral administration of PQ (60 mg/kg). |
| Databáze: | OpenAIRE |
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