Microsatellite Instability in Greek Colorectal Carcinoma Patients: Clinicopathological and Molecular Correlations
| Autor: | Stratigoula Sakellariou, Patroklos Katafygiotis, Irene Thymara, Angelica A. Saetta, Ioanna Giannopoulou, Ilenia Chatziandreou, Penelope Korkolopoulou |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Proto-Oncogene Proteins B-raf Neuroblastoma RAS viral oncogene homolog Oncology Cancer Research medicine.medical_specialty Colorectal cancer medicine.disease_cause High Resolution Melt GTP Phosphohydrolases Metastasis Proto-Oncogene Proteins p21(ras) 03 medical and health sciences symbols.namesake Sex Factors 0302 clinical medicine Internal medicine Humans Medicine Stage (cooking) neoplasms Aged Retrospective Studies Aged 80 and over Sanger sequencing Greece business.industry Age Factors Membrane Proteins Microsatellite instability General Medicine Middle Aged medicine.disease Immunohistochemistry digestive system diseases Sigmoid Neoplasms 030220 oncology & carcinogenesis Colonic Neoplasms Mutation symbols Female Microsatellite Instability KRAS business |
| Zdroj: | Anticancer Research. 39:6379-6387 |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.13851 |
| Popis: | Background/aim In the present retrospective study, we assessed the molecular profile and clinicopathological correlations of Greek colorectal carcinoma (CRC) patients. Patients and methods Data from 157 CRC patients were collected. High Resolution Melting Analysis and Pyrosequencing/Sanger sequencing were applied to identify KRAS, BRAF, NRAS mutations and microsatellite instability (MSI) status. Immunohistochemistry was performed to characterize the associated Mismatch Repair Protein loss. Statistical calculations were performed using the statistical package SPSS v21.0. Results KRAS mutations were detected in 39.3% of cases, BRAF in 10.9% and NRAS in 4.9%. MSI status was recognized in 11.5% of CRC patients and was associated with right colon tumors. MSI phenotype was inversely correlated with stage, N status and KRAS mutations and positively correlated with BRAF mutations. Conclusion MSI positive CRCs in the Greek population are more often right-sided, free of metastasis, KRAS wild type and BRAF mutated. Providing more detailed clinicopathological and molecular data for specific populations will enable better clinical management and individualized therapy in the future. |
| Databáze: | OpenAIRE |
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