Inactivation of Fgf3 and Fgf4 within the Fgf3/Fgf4/Fgf15 gene cluster reveals their redundant requirement for mouse inner ear induction and embryonic survival
| Autor: | Thomas Schimmang, Mark Lewandoski, Hannes Maier, Laura C Zelarayan, Yolanda Alvarez, Iris López-Hernández, Kiril Schimmang-Alonso, Maria Teresa Alonso, Matthew J. Anderson, Victor Vendrell, Elena Domínguez-Frutos |
|---|---|
| Přispěvatelé: | Junta de Castilla y León |
| Rok vydání: | 2021 |
| Předmět: |
animal structures
Fibroblast Growth Factor 3 Fibroblast Growth Factor 4 Nerve Tissue Proteins Biology Fibroblast growth factor Mice Pregnancy Ectoderm FGF4 medicine Animals Inner ear Otic placode FGF15 Embryogenesis Forkhead Transcription Factors Embryonic stem cell Penetrance Cell biology Fibroblast Growth Factors stomatognathic diseases medicine.anatomical_structure Ear Inner Multigene Family Female sense organs Developmental Biology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1097-0177 1058-8388 |
| Popis: | [Background]: Fibroblast growth factors (Fgfs) are required for survival and organ formation during embryogenesis. Fgfs often execute their functions redundantly. Previous analysis of Fgf3 mutants revealed effects on inner ear formation and embryonic survival with incomplete penetrance. [Results]: Here, we show that presence of a neomycin resistance gene (neo) replacing the Fgf3 coding region leads to reduced survival during embryogenesis and an increased penetrance of inner ear defects. Fgf3neo/neo mutants showed reduced expression of Fgf4, which is positioned in close proximity to the Fgf3 locus in the mouse genome. Conditional inactivation of Fgf4 during inner ear development on a Fgf3 null background using Fgf3/4 cis mice revealed a redundant requirement between these Fgfs during otic placode induction. In contrast, inactivation of Fgf3 and Fgf4 in the pharyngeal region where both Fgfs are also co-expressed using a Foxg1-Cre driver did not affect development of the pharyngeal arches. However, these mutants showed reduced perinatal survival. [Conclusions]: These results highlight the importance of Fgf signaling during development. In particular, different members of the Fgf family act redundantly to guarantee inner ear formation and embryonic survival. Consejería de Educación, Junta de Castilla y León, Grant/Award Number: CSI143P20; Programa Estratégico Instituto de Biología y Genética Molecular (IBGM), Escalera de Excelencia, Junta de Castilla y León, Grant/Award Numbers: CCVC8485, CLU-2019-02; MEC, Grant/Award Number: BFU2004-00860/BFI |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text