Identification of a moronecidin-like antimicrobial peptide in the venomous fish Pterois volitans: Functional and structural study of pteroicidin-α
| Autor: | Claude Bouchon, Céline Zatylny-Gaudin, Yolande Bouchon-Navaro, Benoit Bernay, Baptiste Houyvet, Erwan Corre, Didier Goux |
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| Přispěvatelé: | Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), SATMAR, Centre de Microscopie Appliquée à la Biologie [Caen] (CMABio3), Interactions Cellules Organismes Environnement (ICORE), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), ABiMS - Informatique et bioinformatique = Analysis and Bioinformatics for Marine Science (FR2424), Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Fish Proteins
0301 basic medicine Staphylococcus aureus Pterois [SDV]Life Sciences [q-bio] Antimicrobial peptides Peptide Aeromonas salmonicida Aquatic Science Microbiology 03 medical and health sciences Escherichia coli medicine Animals Environmental Chemistry Sea bass chemistry.chemical_classification 030102 biochemistry & molecular biology biology Fishes General Medicine Venomous fish biology.organism_classification medicine.disease Antimicrobial Hemolysis Anti-Bacterial Agents 030104 developmental biology chemistry [SDV.IMM]Life Sciences [q-bio]/Immunology Antimicrobial Cationic Peptides |
| Zdroj: | Fish and Shellfish Immunology Fish and Shellfish Immunology, Elsevier, 2018, 72, pp.318-324. ⟨10.1016/j.fsi.2017.11.003⟩ |
| ISSN: | 1050-4648 1095-9947 |
| DOI: | 10.1016/j.fsi.2017.11.003⟩ |
| Popis: | International audience; The present study characterizes for the first time an antimicrobial peptide in lionfish (Pterois volitans), a venomous fish. Using a peptidomic approach, we identified a mature piscidin in lionfish and called it pteroicidin-α. We detected an amidated form (pteroicidin-α- CONH2) and a non-amidated form (pteroicidin-α-COOH), and then performed their functional and structural study. Interestingly, the two peptides displayed different antibacterial and hemolytic activity levels. Pteroicidin-α-CONH2 was bactericidal on human pathogens like Staphylococcus aureus or Escherichia coli, as well as on the fish pathogen Aeromonas salmonicida, while pteroicidin-α-COOH only inhibited their growth. Furthermore, the two peptides induced hemolysis of red blood cells from different vertebrates, namely humans, sea bass and lesser-spotted dogfish. Hemolysis occurred with low concentrations of pteroicidin-α-CONH2, indicating greater toxicity of the amidated form. Circular dichroism analysis showed that both peptides adopted a helical conformation, yet with a greater α-helix content in pteroicidin-α-CONH2. Overall, these results suggest that amidation strongly influences pteroicidin-α by modifying its structure and its physico-chemical characteristics and by increasing its hemolytic activity. |
| Databáze: | OpenAIRE |
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