Technical feasibility of a murine model of sleeve gastrectomy with ileal transposition
| Autor: | Lee Ying, Geoffrey S. Nadzam, Kathleen A. Martin, Gregory A. Breuer, John Hwa, Matthew O Hubbard |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Blood Glucose
medicine.medical_specialty Sleeve gastrectomy Endocrinology Diabetes and Metabolism medicine.medical_treatment Gene Expression 030209 endocrinology & metabolism Gastroenterology Article Transposition (music) 03 medical and health sciences Random Allocation 0302 clinical medicine Gastrectomy Ileum Internal medicine Gastrins Weight Loss Medicine Animals Peptide YY Cholecystokinin Gastrin Nutrition and Dietetics business.industry Sham surgery Mice Inbred C57BL Disease Models Animal Real-time polymerase chain reaction Feasibility Studies RNA 030211 gastroenterology & hepatology Surgery business Biomarkers Hormone |
| Popis: | BACKGROUND: Sleeve gastrectomy with ileal transposition has been shown to be superior to sleeve gastrectomy alone for promoting weight loss in rat and porcine models. The absence of a mouse model for this procedure has impeded efforts to understand the molecular physiology underlying its efficacy. This study demonstrates the long term survivability of sleeve gastrectomy with ileal transposition in mice. MATERIALS AND METHODS: In this study of technical feasibility, a sleeve gastrectomy with ileal transposition (SGIT), sleeve gastrectomy (SG), or sham surgery (SH) was performed on 7 to 8-week old C57BI/6J mice (n=8 for each). To evaluate long term survivability, mice were placed on an obesogenic diet and weighed weekly for 10 weeks. The intestinal identify of the transposed segment was assessed with gene expression analysis of duodenal-, jejunal-, and ileal-specific hormones using quantitative polymerase chain reaction. RESULTS: Overall, SGIT better prevented weight gain than the SG or sham procedures (10-week postoperative weight: SH: 45.3±1.0 grams, SG: 41.25±1.6 grams, SGIT: 35.4±0.8 grams). Gene expression pattern analysis of three markers of intestinal identity (gastrin, cholecystokinin, and peptide YY) suggest that the ileal identity of the transposed segment is maintained 10-weeks after transposition. CONCLUSIONS: We demonstrate for the first time a reproducible mouse model of sleeve gastrectomy with ileal transposition. Future studies utilizing this model will expand our understanding of the molecular pathways through which the hindgut regulates satiety. |
| Databáze: | OpenAIRE |
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