An in vitro 3D diabetic human skin model from diabetic primary cells

Autor: Kivanc Emre Davun, Deniz Yucel, Sahin Alagoz, Candan Yilmaz Ozdogan, Halime Kenar, Emek Doğer
Rok vydání: 2020
Předmět:
Keratinocytes
Pathology
medicine.medical_specialty
0206 medical engineering
Biomedical Engineering
Bioengineering
Human skin
Biocompatible Materials
02 engineering and technology
Type 2 diabetes
In Vitro Techniques
Models
Biological
Skin Diseases
Umbilical vein
Fibroblast migration
Biomaterials
Diabetes Complications
Imaging
Three-Dimensional
Diabetes mellitus
Materials Testing
medicine
Human Umbilical Vein Endothelial Cells
Humans
Cells
Cultured
Skin
Kidney
Wound Healing
integumentary system
Tissue Scaffolds
business.industry
Metabolic disorder
Hydrogels
Fibroblasts
021001 nanoscience & nanotechnology
medicine.disease
020601 biomedical engineering
Biomechanical Phenomena
medicine.anatomical_structure
Diabetes Mellitus
Type 2
Gelatin
Methacrylates
Collagen
0210 nano-technology
business
Wound healing
Zdroj: Biomedical materials (Bristol, England). 16(1)
ISSN: 1748-605X
Popis: Diabetes mellitus, a complex metabolic disorder, leads to many health complications like kidney failure, diabetic heart disease, stroke, and foot ulcers. Treatment approaches of diabetes and identification of the mechanisms underlying diabetic complications of the skin have gained importance due to continued rapid increase in the diabetes incidence. A thick and pre-vascularized in vitro 3D type 2 diabetic human skin model (DHSM) was developed in this study. The methacrylated gelatin (GelMA) hydrogel was produced by photocrosslinking and its pore size (54.85 ± 8.58 μm), compressive modulus (4.53 ± 0.67 kPa) and swelling ratio (17.5 ± 2.2%) were found to be suitable for skin tissue engineering. 8% GelMA hydrogel effectively supported the viability, spreading and proliferation of human dermal fibroblasts. By isolating dermal fibroblasts, human umbilical vein endothelial cells and keratinocytes from type 2 diabetic patients, an in vitro 3D type 2 DHSM, 12 mm in width and 1.86 mm thick, was constructed. The skin model consisted of a continuous basal epidermal layer and a dermal layer with blood capillary-like structures, ideal for evaluating the effects of anti-diabetic drugs and wound healing materials and factors. The functionality of the DHSM was showed by applying a therapeutic hydrogel into its central wound; especially fibroblast migration to the wound site was evident in 9 d. We have demonstrated that DHSM is a biologically relevant model with sensitivity and predictability in evaluating the diabetic wound healing potential of a therapeutic material.
Databáze: OpenAIRE
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