Enzyme kinetics from circular dichroism of insulin reveals mechanistic insights into the regulation of insulin-degrading enzyme
| Autor: | Claire A. Krasinski, Valerie A. Ivancic, Rebecca J. Meservier, Qiuchen Zheng, Donald E. Spratt, Noel D. Lazo |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Models Molecular Circular dichroism insulin medicine.medical_treatment Biophysics Biochemistry Michaelis–Menten kinetics Insulysin enzyme-substrate interactions protease inhibitor 03 medical and health sciences chemistry.chemical_compound Adenosine Triphosphate Alzheimer Disease Commentaries enzyme kinetics medicine Insulin-degrading enzyme Humans Magnesium Enzyme kinetics metalloproteases Molecular Biology chemistry.chemical_classification Chemistry Insulin Circular Dichroism Cell Biology Small molecule enzyme activity Kinetics 030104 developmental biology Enzyme Diabetes Mellitus Type 2 Proteolysis Commentary Adenosine triphosphate |
| Zdroj: | Bioscience Reports |
| ISSN: | 1573-4935 |
| Popis: | Despite the enormous number of therapeutic advances in medicine, nowadays many diseases are still incurable, mainly due to the lack of knowledge of the pathological biochemical pathways triggering those diseases. For this reason, it is compulsory for the scientific community to investigate and unveil the biomolecular mechanisms responsible for the development of those diseases, such as Alzheimer’s disease and diabetes, which are widespread all over the world. In this scenario, it is of paramount importance to develop new analytical techniques and experimental procedures that are capable to make the above-mentioned investigations feasible. These new methods should allow easy performable analysis carried out in a label-free environment, in order to give reliable answers to specific biochemical questions. A recent paper published on Bioscience Reports by Ivancic et al. (https://doi.org/10.1042/BSR20181416) proposes a new analytical technique capable to reveal some mechanistic insights into the regulation of insulin-degrading enzyme (IDE), a protein involved in the above-mentioned diseases. IDE is a multifaceted enzyme having different and not well-defined roles in the cell, but it is primarily a proteolytic enzyme capable to degrade several different amyloidogenic substrates involved in different diseases. Moreover, many molecules are responsible for IDE activity modulation so that understanding how IDE activity is regulated represents a very challenging analytical task. The new analytical approach proposed by Ivancic et al. reports on the possibility to study IDE activity in an unbiased and label-free manner, representing a valid alternative assay for the investigation of any proteases degradative activity. |
| Databáze: | OpenAIRE |
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