HPLC Separation of Vitamin E and Its Oxidation Products and Effects of Oxidized Tocotrienols on the Viability of MCF-7 Breast Cancer Cells in Vitro
| Autor: | Ina Willenberg, Anne Büsing, Pablo Steinberg, Astrid M. Drotleff, Waldemar Ternes, Michael T. Empl |
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| Rok vydání: | 2015 |
| Předmět: |
Thermal oxidation
Chromatography Cell division Cell Survival Chemistry Tocotrienols Vitamin E medicine.medical_treatment Breast Neoplasms General Chemistry High-performance liquid chromatography In vitro MCF-7 MCF-7 Cells medicine Humans Female Composition (visual arts) General Agricultural and Biological Sciences Oxidation-Reduction IC50 Cell Division Chromatography High Pressure Liquid |
| Zdroj: | Journal of Agricultural and Food Chemistry. 63:8930-8939 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.5b04388 |
| Popis: | Tocotrienols, a vitamin E subgroup, exert potent anticancer effects, but easily degrade due to oxidation. Eight vitamin E reference compounds, α-, β-, γ-, or δ-tocopherols or -tocotrienols, were thermally oxidized in n-hexane. The corresponding predominantly dimeric oxidation products were separated from the parent compounds by diol-modified normal-phase HPLC-UV and characterized by mass spectroscopy. The composition of test compounds, that is, α-tocotrienol, γ-tocotrienol, or palm tocotrienol-rich fraction (TRF), before and after thermal oxidation was determined by HPLC-DAD, and MCF-7 cells were treated with both nonoxidized and oxidized test compounds for 72 h. Whereas all nonoxidized test compounds (0-100 μM) led to dose-dependent decreases in cell viability, equimolar oxidized α-tocotrienol had a weaker effect, and oxidized TRF had no such effect. However, the IC50 value of oxidized γ-tocotrienol was lower (85 μM) than that of nonoxidized γ-tocotrienol (134 μM), thereby suggesting that γ-tocotrienol oxidation products are able to reduce tumor cell viability in vitro. |
| Databáze: | OpenAIRE |
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