Acetaminophen glucuronidation accurately reflects gluconeogenesis in fasted dogs
| Autor: | W. F. Schwenk, J. C. Kahl |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Uridine Diphosphate Glucose
medicine.medical_specialty Physiology Endocrinology Diabetes and Metabolism Urinary system Glucuronidation Carbohydrate metabolism chemistry.chemical_compound Dogs Physiology (medical) Internal medicine medicine Animals Acetaminophen Glycogen Fasting Glucose Endocrinology chemistry Gluconeogenesis Galactose Female Specific activity Biomarkers medicine.drug |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 271:E529-E534 |
| ISSN: | 1522-1555 0193-1849 |
| Popis: | To assess whether acetaminophen glucuronide accurately reflects uridyl diphosphate-glucose (UDP-glucose) derived from gluconeogenesis during fasting, three mongrel dogs received infusions of [U-14C]lactate, [1-13C]galactose, and [6-3H]glucose (after fasting overnight or for 2.5 days). After initiation of the isotopes (3 h), acetaminophen was given, and the urinary acetaminophen glucuronide was isolated. The mean plasma [14C]glucose specific activity (SA) was similar to the mean urinary acetaminophen glucuronide SA both after fasting overnight [299 +/- 19 vs. 296 +/- 14 disintegrations.min-1 (dpm).mumol-1, respectively] and after 2.5 days of fasting (511 +/- 8 vs. 562 +/- 32 dpm/mumol, respectively). Mean plasma glucose flux calculated using [6-3H]glucose decreased (P < 0.05) with two additional days of fasting (18.7 +/- 1.2 vs. 13.6 +/- 0.6 mumol.kg-1.min-1), as did intrahepatic (P < 0.05) UDP-glucose flux measured using [1-13C]galactose (8.6 +/- 0.7 vs. 5.5 +/- 0.3 mumol.kg-1.min-1). We conclude that, in fasted dogs, plasma glucose and UDP-glucose, as sampled by acetaminophen, equally reflect gluconeogenesis and appear to come from the same pool of glucose 6-phosphate. In addition, cycling of glucose moieties through UDP-glucose and glycogen decreases with an increased period of fasting. |
| Databáze: | OpenAIRE |
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