Mutational analyses of neighboring domains of active center of RNase P ribozyme
| Autor: | Yo Kikuchi, Terumichi Tanaka, Tomoaki Ando, Shinnosuke Haga, Naomi Kanda |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Binding Sites
Base Sequence biology RNase P DNA Mutational Analysis Molecular Sequence Data Molecular Conformation Ribozyme General Medicine Cleavage (embryo) Ribonuclease P Active center Structure-Activity Relationship Biochemistry Escherichia coli biology.protein Mammalian CPEB3 ribozyme Binding site Magnesium ion |
| Zdroj: | Nucleic Acids Symposium Series. 48:201-202 |
| ISSN: | 0261-3166 |
| DOI: | 10.1093/nass/48.1.201 |
| Popis: | We prepared series of P2-P4 domain variants of Escherichia coli ribonuclease P (RNase P) ribozyme, and examined enzymatic activities of them. The results indicated that mutations in these domains did not affect the cleavage site selection of the enzyme, but affected cleavage efficiencies and dependence on magnesium ion concentrations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|