A SILVER STAIN FOR DEOXYRIBONUCLEIC ACID
| Autor: | Roy Korson |
|---|---|
| Rok vydání: | 1964 |
| Předmět: |
Silver
Histology Grocott's methenamine silver stain Stain law.invention Silver stain Mice chemistry.chemical_compound law Leukocytes Microtome Animals Perchloric acid Carcinoma Ehrlich Tumor Coloring Agents Methenamine Cell Nucleus Chromatography Staining and Labeling DNA DNA Neoplasm Microtomy Silver nitrate chemistry Silver Nitrate Anatomy Citric acid |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 12:875-879 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/12.12.875 |
| Popis: | By hydrolyzing tissue sections with 1 M citric acid for 30 minutes at 60°C and subsequently treating the sections with methenamine silver for 60 minutes at 60°C (5% silver nitrate in 3% aqueous methenamine), a stain has been developed which is extremely intense and specific for nuclear deoxyribonucleic acid. This has been tested in a number of ways including deoxyribonuclease, trichloroacetic and perchloric acid extraction. The reaction has been successful with fresh frozen cryostat sections as well as with paraffin-embedded material. The stain is likewise satisfactory with tissue fixed in neutral formalin, Carnoy's solution, or formalin-phosphate-sucrose mixture. Because the silver precipitate is precisely localized it may be useful in visualizing chromatin with the electron microscope. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|