Response to FEC Chemotherapy and Oncolytic HSV-1 Is Associated with Macrophage Polarization and Increased Expression of S100A8/A9 in Triple Negative Breast Cancer
| Autor: | Nader El-Sayes, Omar Salem, Karen L. Mossman, Yonghong Wan, Alyssa Vito |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Myeloid medicine.medical_treatment chemotherapy Article Breast cancer breast cancer Medicine tumor microenvironment oncolytic virotherapy RC254-282 Triple-negative breast cancer Tumor microenvironment B cells business.industry Neoplasms. Tumors. Oncology. Including cancer and carcinogens Immunotherapy medicine.disease Immune checkpoint Oncolytic virus Gene expression profiling medicine.anatomical_structure Oncology myeloid cells Cancer research triple-negative breast cancer immunotherapy business |
| Zdroj: | Cancers Volume 13 Issue 21 Cancers, Vol 13, Iss 5590, p 5590 (2021) |
| ISSN: | 2072-6694 |
| Popis: | Simple Summary We have previously reported that a combination of clinical chemotherapies and oncolytic HSV-1 works to sensitize tumors to respond to immune checkpoint blockade. We further showed that this therapeutic platform worked via the upregulation of B cells and the concomitant control of immunosuppressive myeloid cells. In this manuscript, we sought to further dissect the mechanism of myeloid cell regulation and differentiation and to identify a therapeutically driven gene signature that is associated with the switch in the myeloid phenotype. This work not only impacts triple-negative breast cancer but all solid tumor phenotypes as we aim to better understand the underlying immunology associated with responses to immune checkpoint therapies in these typically refractory disease types. Abstract The era of immunotherapy has seen an insurgence of novel therapies driving oncologic research and the clinical management of the disease. We have previously reported that a combination of chemotherapy (FEC) and oncolytic virotherapy (oHSV-1) can be used to sensitize otherwise non-responsive tumors to immune checkpoint blockade and that tumor-infiltrating B cells are required for the efficacy of our therapeutic regimen in a murine model of triple-negative breast cancer. In the studies herein, we have performed gene expression profiling using microarray analyses and have investigated the differential gene expression between tumors treated with FEC + oHSV-1 versus untreated tumors. In this work, we uncovered a therapeutically driven switch of the myeloid phenotype and a gene signature driving increased tumor cell killing. |
| Databáze: | OpenAIRE |
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