Real-time in situ magnetic measurement of the intracellular biodegradation of iron oxide nanoparticles in a stem cell-spheroid tissue model
| Autor: | Laurence Motte, Anouchka Plan Sangnier, Aurore Van de Walle, Luc Lenglet, Yoann Lalatonne, Alexandre Fromain, Claire Wilhelm, Alberto Curcio |
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| Přispěvatelé: | Matière et Systèmes Complexes (MSC (UMR_7057)), Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP) |
| Rok vydání: | 2020 |
| Předmět: |
magnetic nanoparticles
Materials science Magnetism Magnetometer [SDV]Life Sciences [q-bio] Nanoparticle 02 engineering and technology real-time in operando measures 010402 general chemistry 01 natural sciences biodegradation law.invention chemistry.chemical_compound [SPI]Engineering Sciences [physics] magnetometry law stem cells [CHIM]Chemical Sciences General Materials Science Electrical and Electronic Engineering Spheroid 021001 nanoscience & nanotechnology Condensed Matter Physics equipment and supplies Atomic and Molecular Physics and Optics 0104 chemical sciences chemistry Biophysics Magnetic nanoparticles Nanomedicine Stem cell 0210 nano-technology human activities Iron oxide nanoparticles |
| Zdroj: | Nano Research Nano Research, Springer, 2020, 13 (2), pp.467-476. ⟨10.1007/s12274-020-2631-1⟩ |
| ISSN: | 1998-0124 1998-0000 |
| DOI: | 10.1007/s12274-020-2631-1 |
| Popis: | International audience; The use of magnetic nanoparticles in nanomedicine keeps expending and, for most applications, the nanoparticles are internalized in cells then left within, bringing the need for accurate, fast, and easy to handle methodologies to assess their behavior in the cellular environment. Herein, a benchtop-size magnetic sensor is introduced to provide real-time precise measurement of nanoparticle magnetism within living cells. The values obtained with the sensor, of cells loaded with different doses of magnetic nanoparticles, are first compared to conventional vibrating sample magnetometry (VSM), and a strong correlation remarkably validates the use of the magnetic sensor as magnetometer to determine the nanoparticle cellular uptake. The sensor is then used to monitor the progressive intracellular degradation of the nanoparticles, over days. Importantly, this real-time in situ measure is performed on a stem cell-spheroid tissue model and can run continuously on a same spheroid, with cells kept alive within. Besides, such continuous magnetic measurement of cell magnetism at the tissue scale does not impact either tissue formation, viability, or stem cell function, including differentiation and extracellular matrix production. |
| Databáze: | OpenAIRE |
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