Slight Pro-Inflammatory Immunomodulation Properties of Dendritic Cells by Gardnerella vaginalis: The 'Invisible Man' of Bacterial Vaginosis?

Autor: Arlette Tridon, Annie Dosgilbert, Marjolaine Vareille-Delarbre, Marie-Paule Vasson, Julie Falenta, Patrick Brachet, Bertrand Evrard, Christiane Forestier, Thomas Bertran
Přispěvatelé: Unité de Nutrition Humaine (UNH), Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université-Institut National de la Recherche Agronomique (INRA), Université d'Auvergne - Clermont-Ferrand I (UdA), UFR médecine et pharmacie, Facultés de Médecine et de Pharmacie, Faculté de Médecine et de Pharmacie, service immunologie, CHU Clermont-Ferrand, UFR pharmacie, Faculté de Pharmacie, UFR Pharmacie, Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), regional council of Auvergne, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Université d'Auvergne (Clermont Ferrand 1) (UdA), Centre Hospitalier Universitaire de Clermont-Ferrand, Laboratoire Microorganismes : Génome et Environnement - Clermont Auvergne (LMGE), Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Lipopolysaccharides
T-Lymphocytes
Lymphocyte proliferation
medicine.disease_cause
Monocytes
Multiplicity of infection
Immunologie
Immunology and Allergy
Gardnerella vaginalis
maladie inflammatoire
General Medicine
Vaginosis
Bacterial
3. Good health
inflammatory disease
medicine.anatomical_structure
vaginitis
Host-Pathogen Interactions
Vagina
[SDV.IMM]Life Sciences [q-bio]/Immunology
Cytokines
Female
women
Bacterial vaginosis
T-Cells
lactobacillus
metaanalysis
Research Article
lcsh:Immunologic diseases. Allergy
Article Subject
T cell
Immunology
Primary Cell Culture
Biology
Immunomodulation
03 medical and health sciences
Immune system
medicine
Humans
Secretion
Phytohemagglutinins
Cell Proliferation
Mucous Membrane
Dendritic Cells
medicine.disease
Bacterial Load
030104 developmental biology
Cytokine secretion
lcsh:RC581-607
Zdroj: Journal of Immunology Research
Journal of Immunology Research, Vol 2016 (2016)
Journal of Immunology Research, Hindawi Publishing Corporation, 2016, pp.1-13. ⟨10.1155/2016/9747480⟩
Journal of Immunology Research, 2016, pp.1-13. ⟨10.1155/2016/9747480⟩
Journal of Immunology Research, 1-13. (2016)
ISSN: 2314-7156
2314-8861
Popis: The authors thank the CICS of Clermont-Ferrand and are very grateful to Christelle Blavignac, Claire Szczepaniak, and Lorraine Novais Gameiro for their generous cooperation. This work was partially supported by a grant of the regional council of Auvergne; Bacterial vaginosis (BV), the most common genital infection in reproductive-aged women, is associated with increased risk of sexually transmitted infections. Its etiology remains unclear, especially the role of Gardnerella (G.) vaginalis, an anaerobic bacterium characteristic of the BV-alteration of the vaginal ecosystem. In the genital mucosa, dendritic cells (DCs) sense bacteria of the microenvironment via receptors and then orchestrate the immune response by induction of different T cell subtypes. We investigated the interactions between G. vaginalis and human monocyte-derived DCs using a wide range of bacterial concentrations (multiplicity of infection from 0.01 to 100), and the effects of this pathogen on PHA-induced lymphocyte proliferation. As observed by electron microscopy and cytometry, G. vaginalis reduced the internalization ability of DCs by forming extracellular clusters and induced neither DC maturation, nor DC secretion of cytokines, except at the highest dose with a very early DC maturation state. The same profile was observed on lymphocytes with significant increases of proliferation and cytokine secretion only at the highest bacterial concentration. Our findings indicate that G. vaginalis possesses slight immune-stimulating activities against DCs and T cells, reflecting thus a defective inflammatory response and giving rise to the atypical, non-or low-grade, inflammatory clinical disease profile
Databáze: OpenAIRE
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