Development of an Aptamer-Based Lateral Flow Assay for the Detection of C-Reactive Protein Using Microarray Technology as a Prescreening Platform
| Autor: | Thomas Scheper, Rebecca Jonczyk, Ngoc Linh Phung, Cornelia Blume, Johanna Walter, Lisa K. Seiler |
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| Rok vydání: | 2020 |
| Předmět: |
Microarray
Surface Properties Point-of-care testing Aptamer Protein Array Analysis Metal Nanoparticles Biosensing Techniques Sensitivity and Specificity Humans Amino Acid Sequence biology Chemistry C-reactive protein Human patient Collodion General Chemistry General Medicine Flow Cytometry Molecular biology C-Reactive Protein biology.protein Gene chip analysis Ready to use Biomarker (medicine) Gold Aptamers Peptide |
| Zdroj: | ACS Combinatorial Science. 22:617-629 |
| ISSN: | 2156-8944 2156-8952 |
| DOI: | 10.1021/acscombsci.0c00080 |
| Popis: | For improved cost-effectiveness and temperature-stability, a ready to use lateral flow assay (LFA) is developed in this work for detecting inflammation/infection biomarker C-reactive protein (CRP) in human patient samples on the basis of aptamers. In prescreening investigations, an aptamer with CRP affinity was immobilized on microarray chips in forward and sandwich formats to optimize assay conditions. We suggest these microarray techniques as a resource-sparing and fast-screening instrument for evaluation of various conditions. The capability of the aptamer to detect CRP was shown. Optimized assay conditions were consequently transferred to the LFA-platform. Here we could demonstrate for the first time an aptamer-based LFA for the detection of CRP in human patient samples in pathologically relevant concentrations. The cutoff for CRP detection is set at 10 mg/L, providing a distinctive "yes" (≥10 mg/L CRP) or "no" ( |
| Databáze: | OpenAIRE |
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